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《Journal of Nanchang University(Engineering & Technology)》 2010-02
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Isolation and Identification of Several Cellulase-producing Strains and Their Cellulase Productivity Ability

DUAN Xue-hui,YAN Shu-fang,GAO He(State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,China)  
Screened by agar plate containing CMCNa and Congo red indicator,35 cellulase-producing strains were isolated from rotten wood,straw piles and soil sampled in NanChang.Based on their cellulase-producing activities,a rescreening was carried out and 5 cellulase-producing strains possesssing high CMCase and FPA activities were harvested.By the morphology analysis,such as colony,mycelium,spore,these 5 strains were identified as Ramularia NS1,Trichoderma koningii NS2,Penicillium glaucum NS3,White-rot fungi NS4 and T.viride Persex Fx NS5.When cultured in shake flasks,their CMCase activities could reach 297.97 IU/mL,300.11 IU/mL,154.83 IU/mL,146.68 IU/mL,308.14 IU/mL and the FPA activities of 6.56 IU/mL,5.63 IU/mL,4.29 IU/mL,9.63 IU/mL,8.02 IU/mL were achieved,respectively.Under the suitable fermentation conditions(CMC 6 g/L,wheat bran 6 g/L,(NH4)2SO4 4 g/L,at the temperature 30℃ for 3 days),the yields of CMCase and FPAase produced by T.viride Persex Fx NS5 increased 14.26 % and 74.06 % than that of the original activities of 308.14 IU/mL and 8.02 IU/mL.
【Fund】: 江西省科技攻关资助项目(20041B0103000)
【CateGory Index】: TQ925
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