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《Journal of Nanchang University(Natural Science)》 2013-03
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Isolation and cultivation of cells from the tissue of trachea of Rough-legged Buzzard

LI Yao,YUN Fang,SONG Dan,MA Hongmeng,HOU Benlei,LI Yu(Institute of Animal Genetic Resources of Mongolia Plateau Inner Mongolia University,Huhhot,Inner Mongolia,010021,China)  
Tissue culture of cells from trachea tissue of Rough-legged Buzzard and the effects of a series of factors such as medium,serum,insulin,inosine on the cell culture were investigated in the current study.Cells from trachea tissue of Rough-legged Buzzard were obtained successfully by standard tissue culture methods.Four kinds of mediums(DMEM(high glucose),DMEM/F12,M199,MEM alpha) were used during the process of subculture.The result showed that DMEM/F12 was the optimal medium for the cell culture.The cells did not present a serum dependence,but the cell could grow well in the medium containing 10% serum.Certain concentration of insulin and inosine could also promote the cell culture.
【Fund】: 国家科技部农业科技成果转化资金项目(2007GB2A400047)
【CateGory Index】: Q813.1
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