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《Academic Periodical of Farm Products Processing》 2009-03
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Comparative Study on Methods of Extraction and Deproteinization of Crude Polysaccharides in Flatstem Milkvetch Seed

Dong Rui,Qiu Nongxue,Jin Xiaohui (College of Food Engineering and Nutrition Science,Shaanxi Normal University,Xi'an 710062,China)  
The conditions for extracting polysaccharides with water from Flatstem milkvetch seed,including incubation time and temperature,times of extraction and feed liquid ratio,were investigated through an orthogonal design of L9(34)in this work.. The highest yield of 96.9 g/kg was achieved by in the water bath at 65 ℃ for 3 h with the feed liquid ratio at 1∶15,then followed the same process three times. Four methods were used to remove the protein from polysaccharides,and the results showed the combination enzyme and Sevag exhibited higher removal percentage of protein and lower loss of polysaccharide. The proper weight of papain is 0.4 g,the temperature is 45 ℃,time for reaction is 1 h,pH is 7.0,and the times of Sevag to remove protein is 3. After the optium conditions,the removal percentage of protein is 87.5%,and the loss of polysaccharides is 14.9%。
【CateGory Index】: S567.239
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