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《Academic Periodical of Farm Products Processing》 2011-01
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Determination of Six Main Flavonoids in Herba Epimedium Rapidly by High Performance Liquid Chromatography

Jiang Huajun, Li Yinhua, Liu Zhonghua, Huang Zhiqiang, Chang Qiu, Lin Haiyan (National Research Center of Engineering & Technology for Utilization of Functional Ingredients from Botanicals, Hu'nan Agricultural University, Changsha, Hu'nan 410128, China)  
A RP-HPLC method has been established for quickly determination of six main flavonoids in Herba Epimedium. The samples are separated on Welchrom TMC18 (200 mm×4.6 mm, 5 μm) with mobile phase consisted of acetonitrile-water. The column temperature is 35 ℃, the wavelength is 270 nm and the flow rate is 1.0 mL/min. The results show that six Flavonoids reach baseline separation within 27 min. This method has advantages of using little analytic time, a good stability and accuracy of operation, has some references value for establishing standardization quality control method of Epimedium herb.
【Key Words】: high performance liquid chromatography (HPLC) Epimedium Flavonoid
【Fund】: 湖南省科技重大专项基金(07FJ1005)
【CateGory Index】: R284.1
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