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《Renewable Energy Resources》 2011-03
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Comparative study on determination of seed oil content of energy plants by using NMR and Soxhlet Extraction

DONG Xiao-li1,2,BAI Peng-li1,2,WANG Jin-mei1,2,RUAN Cheng-jiang2(1.College of Life Science,Liaoning Normal University,Dalian 116029,China;2.College of Life Science,Dalian Nationalities University,Dalian 116600,China)  
The seed oil content is an important index for evaluating energy plants.To find a quick and precise method for testing the seed oil content,we measured the seed oil contents of four potential energy plants by using the methods of Nuclear Magnetic Resonance(NMR) and Soxhlet Extraction,and compared the results among them.Results showed that the seed oil contents tested by NMR are significantly higher than that of Soxhlet Extraction.For the two methods,the measured seed oil contents of Datura stramonium were 28.87% and 25.91%,respectively;17.74% and 14.78% for Kosteletzkya virginica,respectively;9.53% and 6.82% for Hippophae rhamnoides ssp.sinensis,respectively;and 47.01% and 44.31% for Ricinus communis,respectively.The data indicated that NMR method had the advantages of simple and damage free,which avoided the disadvantages of Soxhlet Extraction such as time-consuming and difficult extraction of fatty acids with combination state(e.g.Lecithin,Glucolipid,Lipoprotein,etc.).Thus,NMR could be used for rapid testing of the seed oil content,which provides a reliable method for screening potential energy plants.
【Fund】: 国家“十一五”科技支撑计划重大项目子专题(2006BAD09A04);; 辽宁省教育厅高等学校科研项目(2009A150);; 中央高校基本科研业务费专项资金(DC10020102)资助
【CateGory Index】: S184
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