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《Modern Agricultural Sciences》 2009-05
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Morchella Handle Crude Protoplast Preparation and Regeneration

FU Xiao-yan 1,FAN Li 2 (1.Weifang Medical College Morphology Laboratory,Weifang Shandong 261053;china;2.College of Life Science,Capital Normal University,Beijing 100048,China)  
The experiment to determine the rough handle morel(Morchella crassipes) the best protoplast preparation conditions,namely: the use of 72 h the mycelium culture to 0.6 mol / L NaCl as a solid preparation for dialysis dose 4 mg / L snail enzyme,5 mg / L of mixed soluble enzyme wall enzyme for cell wall degrading enzyme solution,30 ℃,50 rpm / min digestion 3 h,the highest available output of protoplast 2.07 × 106 months / 100 mg / mL.To 0.6 mol / L NaCl solution so steady infiltration agent,MYG regeneration medium on protoplast regeneration rate to be 0.16%.
【CateGory Index】: S646
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