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《South China Fisheries Science》 2015-06
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Molecular cloning and expression analysis of Ubiquitin-conjugating enzyme gene from black tiger shrimp (Penaeus monodon)

FU Mingjun;ZHAO Chao;ZHOU Falin;QIU Lihua;JIANG Shigui;Key Lab.of South China Sea Fishery Resources Exploitation & Utilization,Ministry of Agriculture;South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences;  
We cloned the complete c DNA sequence of Ubiquitin-conjugating enzyme gene from black tiger shrimps( Penaeus monodon)( Pm Ubc) by rapid amplification of c DNA ends( RACE) methods based on the partial sequence of Pm Ubc obtained from the hepatopancreas transcriptome database. The c DNA of Pm Ubc was 598 bp,including 154 amino acids with an open reading frame( ORF) of 465 bp,17 bp of 5' untranslation region( UTR) and 116 bp of 3' UTR. Blast analysis shows that Pm Ubc shared high identity with Ubiquitin-conjugating enzyme of Litopenaeus vannamei. Real time quantitative PCR analysis reveals that Pm Ubc was constitutively expressed in seven selected tissues( brain,thoracic nerve,heart,hepatopancreas,ovary,muscle,hemolymph). The Pm Ubc mRNA expression reached the maximum at Stage Ⅲ of ovarian development,followed by StageⅠand Ⅳ. It is concluded that Pm Ubc might play an important role in ovarian development of P. monodon.
【Fund】: 现代农业产业技术体系建设专项资金(CARS-47);; 国家自然科学基金项目(31101903);; 广东省科技计划项目(2013B040402016);; 广东省海洋渔业科技推广专项(A201300B03);; 海南省应用技术研发与示范推广专项(ZDXM2014057);; 中央级公益性科研院所基本科研业务费专项资金(中国水产科学研究院南海水产研究所)资助项目(2012TS27 2014TS12)
【CateGory Index】: S917.4
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