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《Journal of Agro-Environment Science》 2008-01
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Isolation of Petroleum-degrading Strains and Their Degrading Characteristics

YANG Xue-lian1, LI Feng-mei2, LIU Wan-ting2, LI Gang2 (1. Shenyang Key Laboratory of Environmental Engineering, Shenyang University, Shenyang 110044, China; 2. Institute of Applied Ecology, CAS, Shenyang 110016, China)  
Petroleum-degrading strains L10 and D6 were obtained by selective enrichment culture from eight contaminated soil samples collected from Liaohe and Daqing Oil Fields. The tests in shaking flasks showed that they could degrade petroleum hydrocarbon well and the removal rates could reach 39.5% and 47.9% at 30 d. According to morphologic observation, physiological and biochemical characteristics, they were identified as Bacillus subtilis and Bacillus licheniformis respectively. Their removal abilities to petroleum were affected by petroleum′s concentrations and properties. At 10 d, the removal rates reduced with increasing concentrations of petroleum. With the treatment time prolonged, the removal rates increased at concentrations 0.5%~2.0% and reduced when the concentrations were 2.0%~10.0%, implying that appropriate petroleum concentration stimulated the activities of microbes, but higher petroleum concentration restrained their activities. The degradation rates of thin-oil by L10 and D6 were higher than those of viscous-oil under the same conditions. The removal rates of aromatics, pectin and asphalt by L10 and D6 were different due to the petroleum properties. The order of removal rate for each component was alkanesaromaticspectin and asphalt, with the removal rates being 62.96% ~78.67%, 16.76%~33.92% and 3.78%~15.22%,respectively. So, it is very important that petroleum properties and microbes characteristics are considered in bioremediation of contaminated soil.
【Fund】: 沈阳环境工程重点实验室资助项目(2004010);; 国家重点基础研究发展计划项目(973)(2004CB418501)
【CateGory Index】: X172
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