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《Journal of Agro-Environment Science》 2014-03
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Characteristics of Microbial Communities in Full-scale Biogas Digesters with Straw as Substrate

ZHANG Lei;LIANG Jun-feng;CUI Wen-wen;DU Lian-zhu;GAO Wen-xuan;Xinmei Feng;Anna Schnürer;Department of Environmental Science and Engineering, Tianjin University;Agro-environmental Protection Institute Ministry of Agriculture;Swedish Institute of Agricultural and Environmental Engineering;Department of Microbiology, Swedish University of Agricultural Sciences;  
In China, low biogas yield and poor system stability have seriously affected the popularization of biogas production with straw as substrate. Understanding microbial processes and their interaction in biogas digesters is a prerequisite to improving efficiency and stability of biogas production. In this study, characteristics of bacterial and archaeal community structures in full-scale biogas digesters with straw as mono or major substrates were studied using terminal restriction polymorphism analysis(T-RFLP)and cloning library construction. The diversity of bacterial community was rich, belonging to nine phyla. Among them, Firmicutes, Proteobacteria and Bacteroidetes were dominant,with the relative abundance of 19.3%~47.2%, 4.8%~24.3% and 2.5%~15.5% respectively. Hydrolytic and fermentative bacteria were dominant population. The species of archaeal population was much less than that of bacterial population, belonging to Methanobacteria and Methanomicrobi. Methanosaeta was the dominant population in the digesters with straw as mono-substrate, and the relative abundance was69.2%~71.9%. However, Methanosarcina was the dominant population with the relative abundance of 73.1% in the digester with straw and pig manure mixture as substrates.
【Fund】: 国家科技支撑项目(2012BAD15B02);; 科研院所技术开发研究专项资金(2011-2013);; 中央级公益性科研院所基本科研业务费专项资金(2011-szjj-ljf);; 天津市科技支撑计划重点项目(12ZCZDNC01800)
【CateGory Index】: S216.4;X712
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【Citations】
Chinese Journal Full-text Database 10 Hits
1 ZHU Wen-xiu,HUANG Zhen-xing,REN Hong-yan,RUAN Wen-quan(School of Environment and Civil Engineering,Jiangnan University,Wuxi 214122,China);Performance and Microbial Community Dynamic Characteristics of an Internal Circulation Reactor Treating Brewery Wastewater[J];Environmental Science;2012-08
2 SHI Hongwei1,ZOU Dexun1,ZUO Jiane2,LIU Yanping1,ZHU Baoning1,LI Xiujin1,,MAO Jingjing1 1. Department of Environmental Science and Engineering,Beijing University of Chemical Technology,Beijing 100029 2. School of Environment,Tsinghua University,Beijing 100084;Investigation on anaerobic digestion performance and microbial structure of vegetable wastes under gradient organic loadings[J];Acta Scientiae Circumstantiae;2012-01
3 ZHANG Wang, LI Xiu-jin, PANG Yun-zhi, CAI Lu-ping ( Beijing University of Chemical Technology, Beijing 100029, China);A Pilot Study on Mesophilic Dry Anaerobic Digestion of Rice Straw[J];Journal of Agro-Environment Science;2008-05
4 SHI Hong-wei1,ZOU De-xun1,ZUO Jian-e2,ZHU Bao-ning1,LIU Yan-ping1,LI Xiu-jin1*(1.Department of Environmental Science and Engineering,Beijing University of Chemical Technology,Beijing 100029,China;2.School of Environment,Tsinghua University,Beijing 100084,China);The Effect of Feedstocks on Microbial Communities in Anaerobic Digesters[J];Journal of Agro-Environment Science;2011-08
5 Bi Yuyun,Wang Yajing,Gao Chunyu(Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences,Beijing 100081,China);Straw Resource Quantity and its Regional Distribution in China[J];Journal of Agricultural Mechanization Research;2010-03
6 Chen Xiaohua1,Zhu Hongguang1,2※(1.Bio-Energy Research Center,Modern Agricultural Science & Engineering Institute,Tongji University,Shanghai 200092,China;2.State Key Laboratory of Pollution Control & Reclamation,Tongji University, Shanghai 200092,China);Research progress and prospect on producing biogas from crop straws[J];Transactions of the Chinese Society of Agricultural Engineering;2007-03
7 QIAO Jiang-tao1,2,GUO Rong-bo1,YUAN Xian-zheng1,SHI Xiao-shuang1,XU Xiao-hui1,FAN Xiao-lei1,QIU Yan-ling1(1.Key Laboratory of Biofuels,Qingdao Institute of Bioenergy and Bioprocess Technology,Chinese Academy of Sciences,Qingdao 266101,China;2.University of Chinese Academy of Sciences,Beijing 100049,China);Phylogenetic Analysis of Methanogenic Corn Stalk Degrading Microbial Communities[J];Environmental Science;2013-04
8 DING Yan-Qin HUANG Wei-Hong YAO Liang-Tong YU Su-Fang DU Bing-Hai*(Department of Microbiology,College of Life Sciences,Shandong Agricultural University,Taian,Shandong 271018,China);Cloning and Functional Analysis of pyrD Gene Involved in Siderophores Biosynthesis in Pseudomonas pseudoalcaligenes B50[J];Microbiology China;2010-07
9 He Zhigang,Niu Shiwei,Yu Tao(Institute of Environmental Resource and Agricultural Energy Liaoning Academy of Agricultural Sciences,Shenyang 110161);Methanogenic Diversity in an Anaerobic Digester with Manure Feces in Low Temperature[J];Chinese Agricultural Science Bulletin;2012-06
10 Cui Wenwen, Liang Junfeng, Du Lianzhu, Zhang Keqiang (Agro-Environmental Protection Institute, Ministry of Agriculture/Key Laboratory of Original Agro-Environmental Quality, MOA, Tianjin 300191);The Current Situation and Problems of the Large-scale Biogas Plants for Straw in China[J];Chinese Agricultural Science Bulletin;2013-11
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 DENG Yi-guo et al (Institute of Agricultural Machinery,Chinese Academy of Tropical Agricultural Sciences,Zhanjiang,Guangdong 524091);Effect of Dry Matter Concentration on Dry Anaerobic Fermentation for Biogas Production in Pineapple Leaf Residues[J];Journal of Anhui Agricultural Sciences;2010-32
2 CHEN Zhao-qiong et al(Department of Public Health,Chengdu Medical College,Chengdu,Sichuan 610083);Study on Rape Straw-Degrading Microbial Community and Degrading Characteristics[J];Journal of Anhui Agricultural Sciences;2011-32
3 LIU Xiu-juan et al (College of Biotechnology and Pharmaceutical Engineering,Nanjing University of Technology,Nanjing,Jiangsu 211816);Study on Biogas Production Using Anaerobic Fermentation of Rice Straw[J];Journal of Anhui Agricultural Sciences;2012-02
4 ZHANG Jin-xing CHANG Zhong-yuan(Agricultural Mechanization Service Center of Zhongwei City in Ningxia Hui Autonomous Region,Zhongwei Ningxia 755000);Research on Comprehensive Utilization of Corn Straw in Zhongwei City[J];Modern Agricultural Science and Technology;2012-08
5 LIU Rui1,WAN Chu-yun1,HUANG Qian1,2,HUANG Feng-hong1(1.Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Wuhan 430062,China;2.Shool of Food Science and Technology,Huazhong Agriculture University,Wuhan 430070,China);Problem and Prospect of Pretreatment of Straw[J];Environmental Science & Technology;2009-05
6 DENG Yi-guo1,WANG Jin-li1,SUN Wei-sheng2,JIAO Jing1,ZHENG Yong1,Wang Gang1(1.Institute of Agricultural Machinery,Chinese Academy of Tropical Agricultural Sciences,Zhanjiang 524091,China; 2.South Subtropical Crops Research Institute,Chinese Academy of Tropical Agricultural Sciences,Zhanjiang 524091,China);Effect of temperature on anaerobic fermentation for biogas production in pineapple leaf residues[J];Guangdong Agricultural Sciences;2010-12
7 ZHU De-wen1,CAO Cheng-mao2,CHEN Yong-sheng1,LI Rui-rong1,QU Hao-li1(1.Nanjing Research Institute for Agriculture Mechanization,Ministry of Agriculture,Nanjing,210014,China; 2.Anhui Agricultural University,Engineering Institute,Hefei,230036,China);Key Technologies and Discussion of the Straw Dry-anaerobic Fermentation[J];Chinese Agricultural Mechanization;2011-04
8 ZHI Qiu-xia,ZHU Jian-liang,TAO Ying(Nanjing University of Technology,Nanjing,210009,China);The Impact of Solid Base on Anaerobic Fermentation[J];Chinese Agricultural Mechanization;2012-02
9 WEI Shi-qing,QIN Wen-neng,JIANG Hu-bo,ZENG Guang-yu,YE Yong-wei(Guangxi Forestry Research Institute,Nanning 530002,China);Study on Dry Anearobic Fermentation of Corn Straw[J];Guangxi Forestry Science;2011-03
10 LU Zhen(Tianhe College,Guangdong Polytechnic Normal University,Guangdong Guangzhou 510540,China);The Study Status and Progress of Anaerobic Fermentation of Water Hyacinth in China[J];Guangzhou Chemical Industry;2012-18
China Proceedings of conference Full-text Database 8 Hits
1 ZHAI Zhi-jun~1 LlJun~2,Chen Xiang-song~2,Wu Jin-yong~2,YAO Jian-ming~1 (1 Key Laboratory of Ion Beam Bioengineering,Chinese Academy of Sciences,Hefei 230031, 2 Anhui Academy of Cyclic Economy and Technology Engineering,Hefei 230088 );Research progress of biomass waste(straw,blue algaeand food waste)utilization[A];[C];2010
2 Zhang Wenqian1, Zhang Yanlin1 (1. College of Engineering and Technology, Huazhong Agricultural University, Wuhan 430070, China);Design of Cutting and Tearing Machine for Rice-wheat Straw[A];Chinese Society of Agricultural Engineering[C];2009
3 Gao Xinxing,Zhao Lixin,Dong Baocheng,Chen Ling,Wan Xiaochun,Luo Juan (Chinese Academy of Agriculture Engineering,Beijing 100125,China);Experimental study on effect of separated two-phase anaerobic fermentation leachate recirculation on anaerobic process[A];Chinese Society of Agricultural Engineering[C];2011
4 Luo Juan,Dong Baocheng,Chen Ling,Gao Xinxing,Wan Xiaochun,Zhao Lixin(Institute of Energy and Environmental Protection,Chinese Academy of Agricultural Engineering,Beijing 100125,China);Biogas-producing characteristics experiments of animal manure and corn straw anaerobic digestion[A];Chinese Society of Agricultural Engineering[C];2011
5 Liu Liang~(1,2),Liu Shengyong~(1,2※),Zhou Huifa~3,Hua Lei~1,Li Yongbing~1 (1.College of Mechanical and Electrical Engineering,He'nan Agricultural University,Zhengzhou 450002,China; 2.Key Laboratory of Renewable Energy of Ministry of Agriculture,Zhengzhou 450002,China; 3.96401 PLA Troops,Baoji 721100,China);Biogas production from peanut vine by anaerobic fermentation[A];Chinese Society of Agricultural Engineering[C];2011
6 Zhu Jin,Ye Xiaomei,Chang Zhizhou,Xu Zhi,Du Jing (Institute of Agricultural Resources and Environment,Jiangsu Academy of Agricultural Sciences,Jiangsu Agricultural Waste Treatment and Recycle Engineering Research Center,Jiangsu 210014,China);Effect of inoculum、pH and aeration on the performance of rice straw in two-phase anaerobic digestion[A];Chinese Society of Agricultural Engineering[C];2011
7 Liang Xiaohui~(1,2),Hua Dongliang~(1,2),Xu Haipeng~(1,2),Zhangjie~(1,2),Liyan~(1,2),Zhang Xiaodong~(1,2) (1.Energy Research Institute of Shandong Acadamy of Sciences,Jinan 250014,China; 2.Key Laboratory for Biomass Gasification Technology of Shandong Province,Jinan 250014,China);Recent research progress of the key technologies for biogas from straws[A];Chinese Society of Agricultural Engineering[C];2011
8 Wang Aijun,Zhang yan,Zhang Xiaotao (Institute of Electric Power,North China University of Water Resources and Electric Power,Zhengzhou 450011,China);Fuel costs analysis of biomass power generation[A];Chinese Society of Agricultural Engineering[C];2011
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Yi-wei(Research & Development Center of Anyang Lipp Silo Engineering Co.,Ltd,Anyang Henan 455004);Research on Anaerobic and Gas Collection of CSTR System in Straw Biogas[J];Modern Agricultural Sciences and Technology;2011-01
2 WU Hongzhong1,DING Xiaojuan1,HU Zhenghu2,ZHANG Weili1 (1.College of Animal Science and Fishery,Anhui Agricultural University,Hefei 230036; 2University of Science and Technology of China);Character of in Vitro Wheat Straw Degradation by Different Microbiology Populations in Rumen[J];Journal of Anhui Agricultural University;2003-03
3 Li Huai1,Guan Weisheng1,Ou Yangerming2,Wang Xiaohui2,Zhang Jie2(1.Department of Environmental Science and Engineering,Chang'an University,Xi'an 710054,China;2.Department of Environmental Science and Engineering,Tsinghua University,Beijing 100084,China);DGGE(Denatured Gradient Gel Electrophoresis) and its Application in the Research of Environmental Microbiology[J];Environmental Science and Management;2008-10
4 ZHAO Mengmeng,JIANG Man,ZHOU Zuowan(Key Laboratory of Advanced Technology of Materials of Ministry of Education,School of Materials Science and Engineering,Southwest Jiaotong University,Chengdu 610031);The Components Analysis of Several Kinds of Agricultural Residues[J];Materials Review;2011-16
5 GONG Weijia,LI Wenzhe,LIU Jianyu(College of Engineering,Northeast Agricultural University,Harbin Heilongjiang 150030,PRC);Progress of research on methanogens bacteria in anaerobic digestion[J];Journal of Northeast Agricultural University;2006-06
6 CHEN Qing-jin~(1,2),LIU Huan-bin~2, HU Yong-you~2 (1. CEPREI Certification Co. Ltd., Guangzhou 510610;2. College of Papermaking and Environmental Engineering,South China University of Technology, Guangzhou 510642);A Continuous Anaerobic Digester for Treatment of Organic Solid Wastes[J];Environmental Science and Technology;2004-03
7 CHU Li-li1,3,LI Yi-bing1,3,FENG Yong-zhong2,3,YANG Gai-he2,3,REN Guang-xin2,3(1.College of Forestry,Northwest A&F University,Yangling,Shaanxi 712100,China;2.College of Agronomy,Northwest A&F University,Yangling,Shaanxi 712100,China;3.Research Center of Recycle Agricultural Engineering and Technology of Shaanxi Province,Yangling,Shaanxi 712100,China);Effect of biogas slurry pretreatment on biogas production characteristics of anaerobic fermentation of wheat straw[J];Agricultural Research in the Arid Areas;2011-01
8 WANG Li1,LI Xue-ming1,XU Yan1(1.College of Urban and Environment,Liaoning Normal University,Dalian 116029,China);The Economic Losses Caused By Crop Residues Burnt in Open Field in China[J];Journal of Arid Land Resources and Environment;2008-02
9 WANG Hai-bo et al(Economy Management College,China Agriculture University,Beijing 100094,China);Utilization,distribution and exploitation tactics of crop stalk resources in China[J];Territory & Natural Resources Study;2008-02
10 YU Fang-fang,WU Jian-dong(College of Environmental Science and Engineering,South China University of Technology,Guangzhou 510006,China);Toxicity Study of Ammonium on Methanogenic Bacteria in Anaerobic Granular Sludge[J];Chemistry & Bioengineering;2008-04
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