Application of ultrafiltration-nanofiltration technology in purification of flavonoids from Cibotium barometz
HAN Wei;JIANG Yishuai;WEI Yutian;Engineering Center for Traditional Chinese Medicine Modernization,School of Pharmacy,East China University of Science and Technology;
The dynamic extraction-ultrafiltration-nanofiltration operation was used to purify the flavonoids in Cibotium barometz. The flavonoids were extracted by the dynamic circulating water solution with the assistance of sodium dodecyl sulfate( SDS),then the macromolecular impurities were removed by ultrafiltration and the feed was concentrated by nanofiltration membrane finally. The ultrafiltration yield of61. 30% and the retention rate of 18. 52% were obtained under the following optimal conditions:ultrafiltration pressure of 0. 14 MPa,temperature of 45 ℃,solution p H of 6,and operation time of 90 min. The nanofiltration yield reached 68. 29% and the retention rate of 53. 92% under nanofiltration pressure of 0. 4 MPa,temperature of 45 ℃,solution p H of 6,and operation time of 90 min with the trapped fluid flavonoid concentration raised to 2. 190 times than the original one. Compared with the nanofiltration and macroporous resin purification,results showed that the succession operation method was time-saving and efficient,and the flavonoids was effectively concentrated.
【CateGory Index】： R284