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《Journal of Naijing University (Natural Sciences)》 2000-05
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SINGLE CELL GEL ELECTROPHORESIS ASSAY IN THE EARTHWORM FOR MEASURING THE GENOTOXITY OF PESTICIDES TO DNA

FENG Shao long, LUO Yu, ZHONG Yuan, XTE chao yun,KONG Zhi ming (State Key Laboratory of Pollution Control and Resource Reuse, Department of Environmental Science and Engineering, Nanjing University,Nanjing, 210093,China)  
In this paper,the DNA damages of earthworms that were caused by pesticides were measured with single cell gel assay,which is an advanced technique for measuring and analyzing DNA breakage in mammalian cell. Earthworms can pass on pollutions between terrigenous and soil organisms,so they can show the changes of soil environment.And it is an important indicator of the safety of pesticides to eco environment.The single cell gel assay of earthworm cell was employed to evaluate the effects of pesticides on eco environment.The results showed two new esticides can damage the DNA of earthworms.We found that the single cell gel assay by earthworm cell can sensitively detect DNA damages that were caused by pesticides.And it can be used as a sensitive indicator of the effects of pesticides on soil.
【Fund】: 国家自然科学基金!(396 70 15 3)
【CateGory Index】: Q75
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