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Construction of S.cerevisiae with Good Osmo-tolerance and High Ethanol-production Characteristic

WU Shuai,CHEN Ye-fu,SHEN Nan and XIAO Dong-guang(College of Bioengineering,Tianjin University of Science and Technology,Tianjin 300222,China)  
185 high ethanol-production haploids and 153 good osmo-tolerant haploids were obtained through spore separation test and spore culture of S.cerevisiae AY-15 and M1 of different properties.Among all the mating types,a type accounted for about 1/4,α type accounted for about 1/2,and others were uncertain strains.By measuring the endurance of yeast haploids,13 high ethanol-production haploids and 19 good osmo-tolerant haploids were selected.After the fermentation,two haploids were selected as the parents for the hybridization.236 diploid strains were constructed by crossing the parents.At last,one strain was obtained through the measurement of their osmo-tolerance capacity and fermenting power and alcohol-resistant test,which could maintain high ethanol-production capacity under hyperosmotic environments.Its ethanol-production capacity in culture medium containing 15 % salt increased by 19.6 % than AY-15 and by 15.4% than M1 respectively.
【Fund】: 天津市重点科技攻关资助项目(06YFGZSH00500)
【CateGory Index】: TS261.1
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