Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Liquor-Making Science & Technology》 2008-04
Add to Favorite Get Latest Update

Research on the Preparation and Regeneration of Protoplast from High-concentration Mash Resistant Saccharomyces cerevisia

YU Zhi-min, LUAN Jing, XU Peng, WANG Ji-hua and ZHAO Chang-xin (Liaoning Key Lab of Fermentation Technology, Dalian Polytechnic University, Dalian, Liaoning 116034, China)  
The preparation and regeneration conditions of protoplast from a high-concentration mash resistant S. cerevisia strain was studied. The results showed that the optimum time for protoplast preparation was the exponential growth of 7 h after the inoculum, and the optimum enzyme for cell wall hydrolysis was snailase. Through L16(5)4 orthogonal experiment, the optimum preparation conditions of S. cerevisia protoplast were identified as follows: 1.0 % of snailase, 0.7mol/L of KCl high osmotic buffer, 1.5 h hydrolysis, 0.1 % of β-mercaptoethanol protectant and hydrolysis temperature at 26 ℃. Under the above conditions, the formation rate and the regeneration rate of protoplast were 80.84 % and 36.03 % respectively. A further study showed that higher regeneration rate (39.78 %) of S. cerevisia protoplast could be obtained when the prepared protoplast was regenerated on the sandwich culture of 7 % sucrose media.
【Fund】: 辽宁省教育厅高校科研基金资助项目(NO2020701093)
【CateGory Index】: TS261.11
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 1 Hits
1 ZHAO Shu-quan1,LIU Yue-cai2 and JIANG Guo-tuo3(1.School of Biological & Food Engineering,Dalian Polytechnic University,Dalian,Liaoning 116034;2.Life Science and Technology College,Heilongjiang August 1st Land Reclamation University,Daqing,Heilongjiang 163319;3.Sanyi Animal Medicine Co.Ltd,Dalian,Liaoning 116036,China);Extraction and Measurement Methods of Coenzyme Q_(10) from Biomass of Saccharomyces Cerevisiae[J];Liquor-Making Science & Technology;2008-11
【Citations】
Chinese Journal Full-text Database 7 Hits
1 Zhang Kechang (Wuxi University of light Industry,Wuxi,Jiangsu 214036,China)[;Fuel Ethanol:Recommendations and Suggestions[J];ENGINEERING SCIENCE;2000-06
2 XIE Lin1 and ZHANG He2(1.Anhui Tejiu Distillery,Suzhou,Anhui 234000,China; 2.Novo Nordisk China Dept,Beijing 100085,China);Some Views on the Investigations in USA[J];LIQUOR-MAKING SCIENCE & TECHNOLOGY;2000-01
3 MA Xiao-yan1,ZHENG Hui2 and LI Ying-jun1 et al.(1.Food Science College of Hebei Agricultural University,Baoding Hebei 071001; 2.Hebei Agricultural University,Baoding,Hebei 071001,China);Breeding of K.Marxianus of High-alcohol-yield by UV-irradiation of Protoplasts[J];Liquor-Making Science & Technology;2006-08
4 WU Shuai, XIAO Dong-guang, YUAN Tong-lei and ZHAO Xu (College of Bioengineering, Tianjin University of Science and Technology, Tianjin, 300222);Selection of Saccharomyces cerevisiae with Good Osmo-, thermo-tolerance and High Ethanol-production Capacity[J];Liquor-Making Science & Technology;2006-09
5 LIN Qiu-ye et al;Comparison of physiological properties between two osmophilic alcohol yeast strains[J];Liquor-making;2003-02
6 CHEN Chun-tao,WANG Sui-lou,WEI Jun(Dept.of Food and Biology Engneering,Zhengzhou Light Industry College,Zhengzhou 450002);Protoplast formation,regeneration and mutagenization ofred yeast producting carotenoids[J];Biotechnology;2002-03
7 WANG Ming-zi, SHI Qiao-qin, ZHOU Xiao-lan, WU Song-gang(Coll. of Bioengineering, Fujian Normal Univ. Fuzhou 350007);Methods to Improve Preparation and Regeneration of Yeast Protoplasts[J];Journal of Microbiology;2005-03
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Cheng-ming et al(Ministry of Education Key Laboratory of Industrial Biotechnology,Jiangnan University,Wuxi,Jiangsu 214036);Study on the Recycling Process of Anaerobic-digested Alcohol Effluent in Cassava Ethanol Fermentation[J];Journal of Anhui Agricultural Sciences;2008-17
2 WU Xianfeng, SUN Bo, CHI Yujie, XU Ning (Food College, Northeast Agricultural University, Harbin 150030, China);Application of high glucose-tolerant yeast to alcohol production by sweet sorghum stalk juice fermentation[J];Journal of Northeast Agricultural University;2008-11
3 Ni Weidou, Li Zheng, Jin Hui (Tsinghua University,Beijing\ 100084, China)[;Thinking Concerning the Utilization of Anhydrous Ethanol From Biomass as Fuel[J];Engineering Science;2001-05
4 YI Yi,WU Shi-hua(Department of Biochemical Engineering,Guangxi University of Technology,Liuzhou 545006,China);Screening of yeast strains with alcohol-tolerance[J];Journal of Guangxi University of Technology;2008-03
5 Liu Jidong,Liu Huixia,Zhou Wenhong, Zhao Chunlin, Yve Junrong (Light Industry and Food Engineering Institute, Guangxi Univ.,Nanning,530004,China);Study on the Inhibited Point of Organic Acid to High Concentration Alcohol Fermentation of Sugarcane Molasses[J];Sugarcane and Canesugar;2006-02
6 LI Xiao-rong,ZENG Ru-yi,LUO Jian,YANG Ming-he*(College of Life Sciences,Fujian Normal University,Fuzhou 350108,China);Preparation and Regeneration of Protoplasts of Penicillium urticae[J];Journal of Fujian Normal University(Natural Science Edition);2009-04
7 ZHENG Zhong-yi~1,XIE Da-ping~1,TAN Zhou-jin~2,XIAO Ke-yu~2,LI Yu-hong~1 (1.College of BioScience and Technology,Hunan Agricultural University,Changsha 410128,PRC;2.Hunan Agricultural University,Changsha 410128,PRC);Influence Factors of Microbial Protoplast Fusion Technology[J];Hunan Agricultural Sciences;2006-04
8 MENG Zhen1,2,ZHANG Xue-cheng2,SHI Yan-xia2(1.Key Laboratory for Sustainable Utilization of Marine Fisheries Resource,Ministry of Agriculture,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Science,Qingdao 266071,China;2.College of Marine Life,Ocean University of China,Qingdao 266003,China);Effects of light intensity and temperature on the growth and pigment accumulation of ultraviolet radiation mutagenized productive Dunaliella salina Mutants[J];Marine Sciences;2008-07
9 Zhu baiming1 Chenyin2 Fu guiming2 1 Environment Engineering Evaluation Center of Environmental Protection Agency of Jiangxi Province,Nanchang, Jiangxi-OAI 330047 2 The key Laboratory of Food Science of MOE of Nanchang University Nanchang, 330047;Developing advancement of fuel alcohol and research current[J];Jiangxi Food Industry;2005-02
10 ZENG Fan-zhou1,JIANG Jian-chun1,WEI Min1,CHEN Yu-ru2(1.Institute of Chemical Industry of Forest Products,CAF;National Engineering Lab.for Biomass Chemical Utilization;Key and Open Lab.on Forest Chemical Engineering,SFA,Nanjing 210042,China;2.College of Life Sciences,Nanjing Normal University,Nanjing 210046,China);Research Progress of Fuel Ethanol Production by Hydrolysis and Fermentation of Biomass[J];Biomass Chemical Engineering;2009-02
China Proceedings of conference Full-text Database 3 Hits
1 ZHANG Pin, WANG Yanli, LIU Zhongdongm, HAN Jie, BAI Hongchao (Henan University of Technology,Henan University of Technology P.O. Box S159, 450052 );Shallowly Discusses on Energy Yielding Techniques of Starch[A];[C];2008
2 Zhao Yinhong Chen Li Wang Honglin Ma Shuxia The School of Chemical & Energy Engineering,South China University of Technology,the Guangdong Provincial Laboratory of Green Chemical Technology,Guangzhou 510640,Guangdong China;Design of Energy Source Ecosystem of Cassava[A];[C];2006
3 Zhou Meijing Wang Jie Xu Xinguo (Food Science and Techniques College of Hebei Agriculture University,Baoding,Hebei 071001,China);Study on producing alcohol by sweet sorghum juice[A];Chinese Society of Agricultural Engineering[C];2007
【Co-references】
Chinese Journal Full-text Database 5 Hits
1 HUANG Wei , XU Jian - zhong , FENG Xiao - Hang(The R&n Center of Juhua Co. Ltd , Hangzhou 310018 , China);Recent Research Progress of Coenzyme Q_10[J];Henan Chemical Industry;2003-02
2 BAI Feng-Yan; LIANG Hui-Yan; JIA Jian-Hua(Systematic Wycologr & Lichenology Laboratory,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100080);EXAMINATION OF COENZYME Q SYSTEMS OF THE CANDIDA STRAINS FROM CHINA BY USING A SIMPLIFIED PROCEDURE[J];MYCOSYSTEMA;2000-02
3 WU Zu-fang, WENG Pei-fang, CHEN Jian ( Faculty of Life Science and Biotechnology, Ningbo University, Ningbo 315211, China; State Key Lab of Industry Biotechnology, Southen Yangtze University, Wuxi 214036, China);Advances of Coenzyme Q_(10) Function Studies[J];Journal of Ningbo University(Natural Science & Engineering Edition);2001-02
4 Hao Suli; Wu Yue; Xu Kangseng(National Institute for the Controt of Pharmaceutical and Biological Products Beijing 100050);Study on the Determination of Coenzyme Q_(10) Content by High Performance Liquid Chromatography[J];CHINESE JOURNAL OF BIOCHEMICAL PHARMACEUTICS;1998-04
5 HAN Shao-ying,DOU Jie,ZHOU Chang-lin(School of Life Science and Technology,China Pharmaceutical University, Nanjing 210009,China);Advances in Coenzyme Q_(10) Production by Microbial Fermentation[J];Pharmaceutical Biotechnology;2006-03
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 Song Yaqiong,Gao Panpan,Lv Le (Department of biological Science and Technology,School of Applied Science,USTB,Beijing 100083,China);Progress of Research on the Extraction Methods of Ubiquinone-1O by Microbial Fermentation[J];Metal World;2009-S1
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 HU Zi bin (Survey, Design and Research Institute of ZPEB,Puyang 457001,China);Normal temperature extraction for the determination of TTC-dehydrogenase activity and its application[J];Industrial Water Treatment;2001-10
2 Qu Qiuhao, Ning Guizan (Agricultural Culture Collection of China, Institite of Soil and Fertilizers, Beijing 100081);Protoplast Formation and Regeneration of Phaffia rhodozyma[J];INDUSTRIAL MICROBIOLOGY;1998-04
3 WANG Yajun 1, YAO Shanjing 1 and WU Tianxing 2 ( 1Department of Chemical and Biochemical Engineering, 2Department of Chemistry, Zhejiang University, Hangzhou 310027,Zhejiang, China);INFLUENCES OF OSMOLYTES ON CELLULAR PROLIFERATION, MORPHOLOGY AND VIABILITY OF Saccharomyces cerevisiae[J];Journal of Chemical Industry and Engineering(China);2004-07
4 Lin Deqiu ( Molecular Biotech Research Center );STUDIES ON PROTOPLAST FUSION OF SACCHAROMYCES CEREVISIAE[J];Journal of South China Normal University(Natural Science);1989-02
5 XIAO Dong-guang, YAO Wen-juan and LI Yu(Tianjin University of Light Industry, Tianjin 300222, China);Theoretical Approach on Recycling Thin Stillage[J];Liquor-making Science & Technology;2001-06
6 WANG Kang-yi,XU Kai-cheng,XIA Pei-yu,LI Qiang and SHAO Rong (Shandong Lanling Meijiu Co.Ltd.,Changshan,Shandong 277731,China);Breeding of Ester-producing Yeast by Mutagenic Treatment[J];LIQUOR-MAKING SCIENCE & TECHNOLOGY;1999-03
7 ZHANG Wei, YUAN Yao-wu, TAN Jian-xin, LI Ying-jun, LIU Jing-wu (Agriculture University of He Bei,Bao Ding 071001,China);Mutagenesis-selection of Saccharomyces cerevisiae which have the better ability of reducing diacetyl by protoplast mutagenization with UV.[J];Liquor-making;2003-02
8 LI Yong-fang,LI Xue-mei,YANG Qing-xiang,LIU Guo-sheng,HE Fang-shu (College of Life Science, Henan Normal University,Xinxiang, 453002,China);Preliminary studies on preparation, regeneration and ultraviolet mutagenesis of protoplast of baker's yeast[J];BIOTECHNOLOGY;2000-02
9 YANG Shi-Hui; FANG Cheng-Xiang; ZHANG Luo-Zhen (College of Life sciences, Wuhan University, Wuhan 430072);A STAINING METHOD USED FOR OBSERVING PROTOPLASTS UNDER THE LIGHT MICROSCOPE[J];MICROBIOLOGY;2000-01
10 Gan Zhlbo et a i.(Laboratory of Food Mierobiology Huazhong Agricultural University Wuhan 430070);THE FORMATION AND REGENERATION OF PROTOPLAST FROM Saccharomyces cerevisiae Ⅻ[J];JOURNAL OF MICROBIOLOGY;1994-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved