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《Liquor-Making Science & Technology》 2011-03
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Investigation on the Application Foreground of Molecular Ecology Technique in the Study of Daqu Microbial Community Diversity

PAN Qin-chun~(1,2),MENG Zhen~1,ZHONG Qi-ding~1,XIONG Zheng-he~1,ZHANG Yu-bin~2 and WU Zhao-zheng~3 (1.China National Research Institute of Food & Fermentation Industries,Beijing 100027;2.School of Life Sciences & Technology of China Pharmaceutical University,Nanjing,Jiangsu 210009;3.Gubeichun Group Co.Ltd.,Wucheng,Shandong 253300,China)  
The application of molecular ecology techniques is of great importance in the study of Daqu microbial community diversity.In this paper, 6 common molecular ecology techniques including DGGE/TGGE,T-RFLP,SSCP,RQ-PCR,LH-PCR and metagenome were introduced and their advantages and disadvantages in the study of microbial community diversity and the research progress in molecular ecology techniques at home and abroad were elaborated.Besides,the application foreground of molecular ecology techniques in the study of Daqu microbial community diversity was also discussed,which could provide technical support in publishing structural diversity and genetic diversity of Daqu microbial communities, assessing dominant bacteria/flora during fermentation,and finding and determining the functional microorganism.
【CateGory Index】: TS262.3
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