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《Journal of Nanjing Forestry Unversity》 2002-06
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Purification and Characterization of Xylanases from Trichoderma reesei

MAO Lianshan1,2,SONG Xiangyang1,YONG Qiang1,YANG Fuguo3,YAO Chuncai1,YU Shiyuan1(1.Nanjing Forestry University,Nanjing 210037,China;2.South China University of Technology,Guangzhou 510541,China;3.Central South University,Changsha 410083,China)  
Two parts of xylanases(Part A and Part B) were separated and purified from a culture filtrate of Trichoderma reesei Rut C30 by ammonium sulfate precipitation,followed by DEAESephadex A50 and SPSephadex C50 column chromatography.Part A and Part B were further purified to homogeneity by Sephadex G100 column chromatography.The molecular weights of Part A and Part B were estimated to be 20 300 and 13 500 by SDSPAGE chromatography.The optimal reaction conditions for Part A and Part B were at 45 ℃,pH 4.5,and at 55 ℃,pH 5.5,respectively.Part A was stable in a pH range from 3.0 to 5.5,while Part B was stable in a pH range from 3.5 to 7.5.The major products of enzymatic hydrolysis with Part A were xylooligosacchrides,with a small amount of xylose,while that with Part B were merely xylooligosacchrides.
【Fund】: 国家自然科学基金资助项目(30070636)
【CateGory Index】: O629
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