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《Journal of Nanjing Forestry University(Natural Sciences Edition)》 2008-03
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The changes of physio-biochemistry and morphological characteristics of Ginkgo biloba L.subjected to high temperature stress

OU Zu-lan,CAO Fu-liang,ZHENG Jun(College of Forest Resources and Environment Nanjing Forestry University,Nanjing 210037,China)  
In order to provide theoretical basis for screening better heat-resistant materials of Ginkgo biloba,two-year-old G.biloba seedlings were respectively treated with the temperatures of 40℃,44℃,48℃ and 28℃(control) for 4 h.The morphological characteristics and the physio-biochemical indexes such as relative electrical conductivity,malondialdehyde(MDA) content,superoxide dismutase(SOD) activity,the contents of ascorbic acid(ASA),soluble protein and soluble sugar were determined.The results showed that G.biloba developed a series of changes in physiology and biochemistry after heat stress,although part of indices changed slightly among 28℃,40℃ and 44℃,but every index ascended sharply from 44℃ to 48℃;morphological characteristics were not influenced when the temperature was below 40℃,but the seedlings withered slightly at 44℃,the results indicated that the changes of physiology and biochemistry were earlier than the changes of morphological characters,the changes of morphological character were the results of physio-biochemical changes;while G.biloba resisting to heat stress,SOD,ASA,soluble sugar and soluble protein played a positive role;there was significant difference about MDA content among under different temperature treatment,as well as the temperature and MDA content had a good linear relationship,so MDA was regarded as the best index for filtering heat-resistant cultivars,whereas for other indexes,they could be used for screening heat-resistant cultivars only with appropriate temperature;there was significant difference for every index between under heat stress of 48℃ and control,when the duration of treatment was 4 h,48℃ was more appropriate temperature for screening heat-resistant cultivars than other temperature.
【Fund】: 国家科技支撑项目(2006BAD18B0301)
【CateGory Index】: S792.95
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