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《JOURNAL OF NANJING FORESTRY UNVERSITY》 1999-04
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RAPD Experiment Protocol and Optimization in Forest Trees

Yin TongmingHan ZhengminHuang MinrenWang MingxiuLi Shuxian (Forest Genetics and Gene Engineering Laboratory Nanjing Forestry UniversityNanjing210037)  
RAPD marker is the most widely adopted molecular marker in forest genetic studies. The main reason is that this kind of markers can be obtained without any DNA sequence information about the studying material before hand. So it is especially suitable for the organism which little molecular study has been carried on. We can design primers arbitratily according to a common rule and the experiment process is relatively simple compared with other kinds of markers, such as RFLP and SSR. But this kind of marker has a fatal shortcoming, if the reaction condition is not suitable for the studying material, it gives out very flexible bands and cant be repeated. So the suitable amplification condition is critical to RAPD analysis. In this paper, the influencing factor on RAPD experiment was analyzed, such as the annealing temperature, the Mg2+ 2]concentration, the template concentration and so on. An optimized protocol especially suitable for forest trees was put forward through a series of experiments. According to the protocol, the RAPD condition can be fast determined for different material and the RAPD bands can be well repeated.
【Fund】: 国家自然科学基金
【CateGory Index】: S722.3
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【References】
Chinese Journal Full-text Database 10 Hits
1 QUE Sheng-quan et al(Department of Biology and Environmental Engineering,Nanchang University of Technology,Nanchang,Jiangxi 330013);DNA Extraction from Camellia oleifera and the Establishment of the Optimum Reaction System of RAPD Analysis[J];Journal of Anhui Agricultural Sciences;2008-03
2 XU Jian-peng et al(College of Life Science,Ludong University,Yantai,Shandong 264025);DNA Extraction from Kareius bicoloratus Genome and Its Optimization of RAPD Reaction System[J];Journal of Anhui Agricultural Sciences;2008-35
3 Jiang Yuanbiao(Forestry Research Institute of Simao Prefecture,Yunnan Province,Simao 665000,P.R.China);Wu Tao,Chen Shaoyu,Yuan Ruiling(the Key Laboratory of Forest Plant Cultivation and Utilization of Yunnan Province,Yunnan Academy of Forestry);Optimization of RAPD Reaction Conditions for Pinus kesiya var. langbianensis[J];Journal of Northeast Forestry University;2007-09
4 LI Jun-min, KE Shi-sheng, JIN Ze-xin ( Department of Biology and Chemistry, Taizhou Teachers` College, Linhai 317000, China );Extraction and determination of DNA from Heptacodium miconioides[J];Guihaia;2002-06
5 YAN Dong-hai1,ZHENG Dao-jun2,LIANG Yuan-fa 1,LINGHU Chang-di1,LIU Guo-min2,TIAN Yong-hui1(1.The Tea Research Institute of Guizhou Province,Guizhou,Meitan 564100;2.The kudingcha Research Institute of Hainan University,Hainan,Haikou 570228 );STUDY ON THE OPTIMIZATION OF RAPD-PCR EXPERIMENTAL SYSTEM IN LIGUSTRUM ROBUSTUM (ROXB) BL[J];Guizhou Science;2007-02
6 MAO Yun-ling et al (Yunnan Academy of Forestry,Yunnan Key Laboratory for the Protection and Propagation of Rare and Endangered Forest Plants of State Forestry Bureau,Key Laboratory for the Cultivation and Exploitation of Forest Plants in Yunnan Province,Kunming,Yunnan 650204);Establishment of ISSR-PCR Reaction System for the Rare and Endangered Plant Manglietia grandis Hu et Cheng[J];Journal of Anhui Agricultural Sciences;2009-31
7 HOU Wan-wei1,FAN Jun-feng1,ZHANG Xiao-juan2,LI Xue-lin3(1.College of Forestry,Northwest Agriculture and Forestry University,Yangling 712100,China;2.College of Plant Protect,Northwest Agriculture and Forestry University,Yangling 712100,China;3.Qinghai Huangyuan Forestry Bureau,Huangyuan 812100,China);Optimization of RAMP Reaction System in Poplar[J];Acta Agriculturae Boreali-Sinica;2008-S2
8 WANG Hai-yan1,WANG Yu-guo2,LU San-kuang-sheng1(1.Department of Bio-engineering,Shuozhou vocational technical college,Shuozhou 036002,China;2.College of Agronomy,Shanxi Agricultural University,Taigu 030801,China);Method to Genomic DNA Extraction and RAPD System Establishment in Z.jujuba[J];Natural Science Journal of Hainan University;2008-04
9 ZHANG Ri qing, HE Fang, LU Fang de, LI Bin (Research Institute of Nontimber Forest Crops, South Central Forestry University,Zhuzhou, Hunan 412006, China);Population Genetic Analysis by Randomly Amplified Polymorphic DNA Markers in Pecan[J];Economic Forest Reseaches;2001-02
10 ZHANG Ri qing, TAN Xiao feng, Lü Fang de (Ministerial Key Lab of Economic Forest Tree Breeding and Cultivation, South Central Forestry University, Zhuzhou 412006, Hunan China);RAPD Marking and Its Application in Forest Genetics and Tree Breeding[J];Journal of Jishou University(Natural Science Edition);2001-03
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Xu Haixia,Zhang Xia,Yan Ping,Du Jinzhou(College of Biological Engineering,Shihezi University,Shihezi,Xinjiang 832003,China);A Studies on DNA Extraction and Establishmen of RAPD Reaction System in Achnatherum splendens[J];Anhui Agricultural Science Bulletin;2007-09
2 Xu Liuyi et al (Anhui Province Resistance Breeding for Pine Nemotode Centre,Hefei,Anhui 230036);Study on the Variation of the Outer Form Character of Pinus taiwanensis[J];Journal of Anhui Agricultural Sciences;2004-05
3 YANG Jun-bao et al(Sichuan Provincial Key Laboratory of Environmental Science and Biodiversity Conservation,China West Normal University,Nanchong,Sichuan 637000);Optimization of RAPD Maker in Pinellia ternata[J];Journal of Anhui Agricultural Sciences;2006-02
4 HAN Ling et al(College of Horticulture,Shenyang Agricultural University,Shenyang,Liaoning 110161);Optimization of RAPD-PCR Reaction System of Lilium lancifolium Thunb.Using Orthogonal Design[J];Journal of Anhui Agricultural Sciences;2007-17
5 WANG Yun et al(Special Vegetable Research Institute,Hunan University of Arts and Science,Changde,Hunan 415000);Effects of the Different Fertilization Levels on the Growth and Development,and Prophase Yield of Dolichos lablab L.[J];Journal of Anhui Agricultural Sciences;2007-21
6 LI Hai-li et al(National Research Center of Floriculture Engineering,Beijing Forestry University,Beijing 100083);Establishment of the Optimum Reaction System for ISSR-PCR of Hemerocallis fulva L.[J];Journal of Anhui Agricultural Sciences;2008-12
7 JIA Wen-jie et al (College of Life Science,Nanchang University,Nanchang,Jiangxi 330031);Extraction of Genomic DNA and Optimization of ISSR Reaction Condition for Aquilaria sinensis(Lour.) Gilg[J];Journal of Anhui Agricultural Sciences;2008-24
8 ZHAO Yang et al (College of Forestry,Guizhou University,Guiyang,Guizhou 550225);Establishment of DNA Extraction and ISSR-PCR Reacting System of Tutcheria kweichowensis[J];Journal of Anhui Agricultural Sciences;2008-29
9 CHAI Su-fen et al(Department of Life Sciences,Huizhou University,Huizhou,Guangdong 516007);Construction and Optimization of ISSR Reaction System of Medicinal Rutaceae Plants[J];Journal of Anhui Agricultural Sciences;2008-33
10 MA Li-ya et al (College of Life Sciences,Nanchang University, Nanchang,Jiangxi 330031);Establishment and Optimization of ISSR-PCR Reaction System for Cybidium goeringii[J];Journal of Anhui Agricultural Sciences;2008-34
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 Cao Qingqin1 Zhen Zhen2 Jiang Jie1 Liu Yufen1 Qin Ling1(1Department of Plant Science and Technology;2Department of Biotechnology,Beijing Agricultural College,Beijing 102206,China);Application of Molecular Markers in Castanea[J];Anhui Agricultural Science Bulletin;2007-11
2 LI Rui-huan et al(College of Forestry,Northwest A&F University,Yangling,Shaanxi 712100);Genomic DNA Extraction and RAPD System Optimization of Chinese Jujube[J];Journal of Anhui Agricultural Sciences;2007-17
3 LIU Yun-hua et al(National Gaofeng Forest Farm of Guangxi Autonomy District,Nanning,Guangxi 530004);Research Advance in the High-yielding Cultivation Technique of Polygonum multiflorum Thunb[J];Journal of Anhui Agricultural Sciences;2007-18
4 MO Zhao-zhan et al (Yulin Normal College, Yulin, Guangxi 537000);Study on Genetic Diversity of Male Ginkgo biloba L[J];Journal of Anhui Agricultural Sciences;2007-26
5 ZHOU Yu-bi et al (Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining,Qinhai 810001);Effect of BSA on RAPD Analysis of Cynomorium songaricum[J];Journal of Anhui Agricultural Sciences;2007-28
6 XUE Zhi-zhong et al(College of Biological Science and Engineering,Hebei University of Science and Technology,Shijiazhuang,Hebei 050018);DNA Extraction from Oil Sunflower Seeds and the Optimization of Its RAPD Reaction System[J];Journal of Anhui Agricultural Sciences;2008-06
7 XU Jian-peng et al(College of Life Science,Ludong University,Yantai,Shandong 264025);DNA Extraction from Kareius bicoloratus Genome and Its Optimization of RAPD Reaction System[J];Journal of Anhui Agricultural Sciences;2008-35
8 LI Jun-min,JIN Ze-xin(Ecology Institute of Taizhou University,Linhai 317000);Optimization and screening of ISSR reaction system in endangered plant Emmenopterys henryi[J];Journal of Anhui Agricultural University;2006-04
9 GUI Teng-qin1,2,3,QIAO Ai-min3,SUN Min1,WANG Xin-yan3,SUN Xue-mei1(1.College of Life Science,Southwest University,Chongqing 400715,China;2.Dept.of Chemistry & Life Science,Qianxinan Teachers' College,Xingyi,Guizhou 562400,China;3.College of Agriculture and Landscape Architecture,ZhongKai University of Agriculture and Technology,Guangzhou,Guangdong 510225,China);Comparison of Extraction Methods and ISSR Analysis of Genomic DNA in Japanese apricot(Prunus mume Sieb.et Zucc.)[J];Northern Horticulture;2008-04
10 WANG Yi,CHEN Chong-shun,CAO Wei-jie,WANG Zhuan-mei(Jiangsu Key Laboratory for Biodiversity & Biotechnology,College of Life Sciences,Nanjing Normal University,Nanjing 210046,China);Establishment of Eustoma grandiflorum Optimal RAPD Reaction System Using Orthogonal Design[J];Northern Horticulture;2008-05
China Proceedings of conference Full-text Database 4 Hits
1 Li Jianchao Liu Mengjun~* (Research Center of Chinese Jujube,Agricultural University of Hebei,Baoding China 071001);Application of RAPD and AFLP Markers on Dry Fruit[A];[C];2005
2 Jiang Xiangmei Wen Qiang Ye Jinshan Xiao Fuming Jiang Mei (Jiangxi Provincial Bio-tech Key Lab for Plant,Nanchang 330032);Population Genetic Diversity Analysis by RAPD in Phoebe bournei[A];[C];2006
3 CAO Liang~1,LI Shun-xiang~(1,2*),WEI Bao-yang~1,HUANG Dan~2,XU Fei~1,Tong Zhi-yuan~1 (1 College of Pharmacy,Hunan University of Traditional Chinese Medicine,Changsha 410208,China;2 Hunan Academy of Traditional Chinese Medicine,Changsha 410006,China;3 College of Bioscience and Technology,Hunan Agricultural University,Changsha 410128,China);Modifing RAPD Reaction system and authentic Genuineness related genetic background of Evodia rutaecarpa[A];[C];2009
4 Wu Qinghua Dong Qingsong (Guangxi Botanical Garden of Medicinal Plants,Nanning 530023);Research Survey of Artificial Cultivation of Polygonum multiflorum Thunb.[A];[C];2009
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