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《JOURNAL OF NANJING AGRICULTURAL UNIVERSITY》 1999-03
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Preliminary research on the diagnosis of chicken infectious anemia by PCR,dot blot hybridization assay and IFA

Sun Wei 1,Wu Zhongqin 1,Hu Qinghai 2,Li Shuxiang 3 and Li Gang 1 ( 1 Key Lab of Animal Disease Diagnostic & Immunology, Ministry of Agriculture,Nanjing Agric Univ,Nanjing 210095; 2 Jiangsu Poultry Research Institute,Yangzhou 225007; 3 Statio  
The chicken anemia virus(CAV)was detected by dot blot hybridization assay using the 0 85 kb PCR products labeled with Digoxigenin as probes.The special fragments(0 58 kb)of CAV DNA were amplified by the polymerase chain reaction(PCR).Using the two methods the tissue DNA samples of Jiangsu Province were detected and the same results showed that 6 out of 20 chicken flocks were positive(the positive rate was 30%).For the serums of the same samples,7 out of 20(35%)were positive by the indirect immunofluorescence assay(IFA).The result of IFA compared with the PCR/dot bloting showed 83% consistency.The preliminary investigation showed that the CAV infection is prevalent in the areas of Jiangsu Province.
【Fund】: 农业部 “九五”攻关课题
【CateGory Index】: S858.310.53
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【References】
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1 JIANG Ling yan,WEI Ping,LI Yi jie (Institute of poultry science & health,Guangxi University,Nanning,Guangxi,530005,China);Molecular Biological Characteristic of Chicken infectious anemia virus and its Detection[J];Progress In Veterinary Medicine;2003-05
【Co-references】
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1 SUN Su-ling(Anhui Science and Technology University,Fengyang,Anhui 233100);Development of Colloidal Gold Rapid Diagnostic Stripe against Chicken Infectious Anaemia[J];Journal of Anhui Agricultural Sciences;2008-12
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【Secondary References】
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1 SONG Jian-ling1,SHI Feng-hai2,TIAN Jian-guo3,YE Cong-hua3,ZHANG Wen-dong4,ZHAO Huan-yun4,LU Yue4,YUE Liang4,LI Hua-chun1,QIU Wei5,FENG Zi-iang5,ZHANG Si-dong4,FAN Quan-shui5,ZHANG Ying-guo4,ZHANG Fu-qiang5 (1.Yunnan Tropical and Subtropical Animal Virus Diseases Laboratory,Kunming,Yunnan,650224,China;2.Centre for Animal Disease Control and Prevention of Lincang,Lincang,Yunnan,677000,China;3.Yunnan Agriculture University,Kunming,Yunnan,650223,China;4.Centre for Animal Disease Prevention and Control,Yunnan province,Kunming,Yunnan,65001,China;5.Centre for Disease Prevention and Control,Cheng Du Military Region,Kunming,Yunnan,650032,China);Detection of Mixed Infections of AIV,NDV and Three Kinds of Immunosuppressive Viruses in Yunnan Province[J];Progress in Veterinary Medicine;2010-08
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