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《Journal of Nanjing Agricultural University》 2005-02
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Protective effect by oral administration of α_2-macroglobulin on Xiphophorus helleri

LI Feng-ling,LU Cheng-ping* (Key Laboratory of Animal Disease Diagnostic and Immunology,Ministry of Agriculture, Nanjing Agricultural University,Nanjing 210095,China)  
Aeromonas hydrophila J-1 strain was cultured in LB medium,at 28 ℃ for 24 hours.After centrifugation and inactivated the whole bacteria and the toxin vaccine were prepared.α2-macroglobulin(α2M) were purified from grass carp plasma.The feeds were manufactured containing the whole bacteria,the toxin,the whole bacterium with α2M,and the toxin with α2M respectively,as well as the common feed as the control.150 Xiphophorus helleri were divided into five groups,with 30 fish per group.The above feeds were fed to experiment fishes respectively for successive 15 days and common feed for further successive 20 days.All five groups were challeged with AhJ-1 strain 50 LD 50. The whole bacteria vaccine had the relative percentage survival(RPS)of 43.3%,the whole bacteria vaccine with α2M 53.3%,the toxin vaccine 26.7%,the toxin vaccine with α2M 30%,while the negative control fishes died completely.χ2 test showed that the difference between the immunized groups and the control group was significantly.The difference between the groups with or without α2M was not obviously but α2M could delay the death time of Xiphophorus helleri.
【CateGory Index】: S948
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