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《Journal of Nanjing Agricultural University》 2007-02
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Identification of Parthenocissus tricuspidata endophyte and characterization of its glutamate decarboxylase

YANG Sheng-yuan1,2,LU Zhao-xin1,SUN Li-jun1,L Feng-xia1,BIE Xiao-mei1,LIU Zhan-min1(1.College of Food Science and Technology,Nanjing Agricultural University,Nanjing 210095,China;2.College of Life Science and Technology,Guangxi University,Nanning 530005,China)  
One endophytic bacterium EJC-1 producing glutamate decarboxylase(GAD)was screened from 5 strains isolated from Parthenocissus tricuspidata(Sieb.et Zucc.)Planch.When the biotransformation was conducted with the ratio of wet cells and 10 g·L-1 L-glutamate at 1∶10(W∶V)under 120 r·min-1 at 30 ℃ for 24 h,the γ-aminobutyric acid of biotransformation solution of strain EJC-1 was(3.07±0.23)mmol·L-1.Base on morphological,physiological and biochemical characteristics,16S rDNA and phylogenic analysis,the strain EJC-1 was classified to Bacillus megaterium.The properties of its GAD were also examined,and the optimal temperature and pH for GAD activity were 50 ℃ and 5.6,respectively.The GAD activity was stable at pH 5-6 and below 40 ℃.2.5 mmol·L-1 Mg2+ significantly increased GAD activity,which promoted 13.85% of the activity.
【CateGory Index】: Q93
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