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《Journal of Nanjing Agricultural University》 2007-04
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Characterization of rDNA and detection of Meloidogyne hispanica

HU Yong-jian,WANG Xuan,LI Hong-mei*(Key Laboratory of Monitoring and Management of Diseases and Insects,Ministry of Agriculture,Nanjing Agricultural Univisity,Nanjing 210095,China)  
The internal transcribed spacers(ITS)and intergenic spacer 2(IGS2)of rDNA were amplified and the PCR products were sequenced from populations of Meloidogyne hispanica.Based on the differences between the sequences of M.hispanica,M.incognita,M.javanica,M.arenaria,and M.hapla,RFLP was carried out on the PCR products of IGS2 region by Taq Ⅰ,and a specialized 554 bp fragment from M.hispanica can distinguish it from the other four Meloildogyne species.Reliability and sensitivity of IGS2-RFLP can be achieved from the single juvenile.
【Fund】: 国家自然科学基金项目(30471141);; 国家863计划项目(2001AA249021)
【CateGory Index】: S432.45
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 MENG Qing-peng,LONG Hai,XU Jian-hua* (Key Laboratory of Monitoring and Management of Diseases and Insects, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China);PCR Assays for Rapid and Sensitive Identification of Three Major Root-Knot Nematodes, Meloidogyne incognita, M. javanica and M. arenaria[J];Acta Phytopathologica Sinica;2004-03
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 HUANG Jia-de (Guangxi Institute of Botany,Guilin,Guangxi,541006,China);Control of Major Diseases and Pests in Siraitia grosvenorii[J];Journal of Guangxi Academy of Sciences;2006-03
2 GUO Zhen~1,CHEN Shao-ping~2,CHEN Yong-hui~1,LIAO Jin-Ling~3(1.Guangzhou Agricultural Standard and Supervisory Center,Guangzhou 510308,China; 2.Guangzhou Agricultural Environmental and Plant Protection Station,Guangzhou 510405,China; 3.College of Resources and Environmental Sciences,South China Agricultural University.Guangzhou 510642,China);Primary identification and control of some ornamental plant parasites nematode in Guangzhou[J];Guangdong Agricultural Sciences;2006-02
3 SHU Jing et al(Anhui Agricultural University,Hefei,Anhui 230036);Effect of Environment Factor on Crop Root Nematode Community and Fluctuation[J];Journal of Anhui Agricultural Sciences;2006-06
4 ZHOU Yin-li et al(Department of Agronomy,Yunnan Honghe College,Mengzi,Yunnan 661100);Identification of Root-rot Nematodes in Megranate in Yunnan Province[J];Journal of Anhui Agricultural Sciences;2007-32
5 CHEN Xiao-ling1,LI Xiao-ni2,GAO Yong-feng3,FANG Yu-sheng3(1.Gaoming Enter-exit Inspection and Quarantine Bureau,Gaoming 528500,China; 2.Plant Protection Station of Guangdong,Guangzhou 510500,China; 3.College of Resources and Environment,South China Agricultural University,Guangzhou 510640,China);Study on methoddogy of morphological identification of Pratylenchus coffea parasitising in banana in Guangzhou[J];Guangdong Agricultural Sciences;2008-02
6 FENG Gui-Fang1, LIU Wei-Zhi21.School of Life Science of Linyi Normal University,Shandong Linyi 276005 China2.Department of Plant Protection Shenyang Agricultural University, Liaoning Shenyang 110161,China;DESCRIPTION OF A NEW SPECIES, THE GENUS APHELENCHOIDES NEMATODE[J];Acta Zootaxonomica Sinica;2008-02
7 ZHOU Yin-li et al (Department of Agronomy,Honghe College of Yunnan Province,Mengzi,Yunnan 661100);Study on the Species of Parasitic Nematodes in the Rhizosphere of Punica granatum Linn.[J];Journal of Anhui Agricultural Sciences;2008-04
8 XIE Zhi-cheng,LU Wei-cheng,YANG Qing,CHENG Jing,ZHANG Shao-sheng(College of Plant Protection,Fujian Agricultrue and Forestry University,Fuzhou,Fujian 350002,China);Eight species of nematodes parasitized at the roots of rice[J];Journal of Fujian Agriculture and Forestry University(Natural Science Edition);2007-01
9 WANG Xin-rong1,HU Yue-qing1,ZHU Xiao-wei1,ZHONG Tian-kui2,ZHUO Kan1,KONG Xiang-chao1(1.College of Natural Resources and Environment,South-China Agricultural University,Guangzhou,Guangdong 510642,China;2.Forest Pest Management and Quarantine Station of Guangzhou,Guangzhou,Guangdong 510030,China);Nematodes specie survey and identification in pine wood of Pinus massoniana in Guangdong Province[J];Journal of Fujian College of Forestry;2008-01
10 HAN Xin-hua1,2,XU Yan-li1,PAN Feng-juan1,2,and Li Chun-jie1(1 Northeast Institute of Geography and Agroecology,Chinese Academy of Sciences,Harbin 150081,Heilongjiang;2Graduate School of the Chinese Academy of Sciences,Beijing 100049,China);Vertical Distribution of Nematodes in Soybean Field in Black Soil Region[J];Soybean Science;2008-02
【Co-references】
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1 CHEN Chun~(1,2),XIE Hui~1,WEI Ying-jun~1,HAN Yu-chun~1,QIN Dan~1,HUANG Chun-xiao~1,TANG Jiong~1(1 Lab of Plant Nematology,South China Agric.Univ,Guangzhou 510642,China;2 Division of Science and Technology Management,South China Agric.Univ.,Guangzhou 510642,China);Studies on Cultural Technique and Reproduction Fitness of Radopholus similis[J];Journal of South China Agricultural University;2006-01
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5 GAN Yan ZENG Fan Ya ZHAO Yun WANG Mao Ling (College of Life Science, Sichuan University, Chengdu 610064, China);Development of SCAR Markers to Authenticate the Hybrid Seed Lots of Shuza No.6( Brassica napus L.)[J];Acta Agronomica Sinica;2001-06
6 JIANG Li-qin,ZHENG Jing-wu(Institute of Biotechnology,College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310029,China);Characterization of the D2-D3 expansion regions of 28S RNA gene in some Bursaphelenchus species[J];Acta Phytopathologica Sinica;2007-06
7 GE Jian- jun, CAO Ai-xin, LIU Xian-bao, CHEN Chang-fa (Chinese Academy of Quarantine and Inspection, Beijing 100029,China; South China University of Tropic Agriculture, Hainan 571737,China;Shandong Inspection and Quarantine Bureau , Qingdao 266002, China);Quantity assay of Bursaphelenchus xylophilus with TaqMan-MGB probe[J];Acta Phytopathologica Sinica;2005-S1
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9 Peng De liang 1, S. Subbotin 2, M.Moens 3 ( 1Institute of Plant Protection of Chinese Academy of Agricultural Sciences, Beijing 100094, China; 2Institute of Parasitology of Russian Academy of Sciences, Leninskii Prospect 33, Moscow 117071, Russia; 3Agriculture Research Center, Crop Protection Department, Burg.Van Gansberghelaan 96, 9820 Merelbeke, Belgium);rDNA restriction fragment length polymorphism of Heterodera avenae in China[J];Acta Phytopathologica Sinica;2003-04
10 Wang Chong,Ge Jianjun(Institute of Animal and Plant Quarantine,CAIQ,Beij ing 100029),Chen Changfa(Qingdao Entry-Exit Ianspection and Quarantine Bureau);Detection of Ditylenchus dipsaci by real time PCR.[J];Plant Quarantine;2005-01
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