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《Journal of Nanjing Agricultural University》 2008-03
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Inhibition of growth and aflatoxin production of Aspergillus flavus by Lactobacillus curvatus HB02

CAO Dong-mei1,ZHANG Hong-ying2,HE Cheng-hua1,JING Sheng1,ZHANG Hai-bin1*(1.College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China;2.Nanjing Center for Disease Control and Prevention,Nanjing 210003,China)  
Lactobacillus curvatus HB02,which could inhibit growth of fungi,was cultured on doubled-layered agar or in liquid anaerobic cultivation medium.The content of volatile fatty acid(VFA)or lactic acid in cultural medium was measured by gas chromatography or colorimeter.The inhibition of HB02 on growth and toxin generation of Aspergillus flavus was determined by co-culture of HB02 with the fungi.The results showed that HB02 grew faster in MRS liquid medium than in solid plate.And in the cultural medium,the content of acetic acid,propionic acid,butyric acid and lactic acid was 51.75 mmol·L-1,0.77 mmol·L-1,0.37 mmol·L-1,and 28.06 mmol·L-1,respectively.The further experiment showed that the more lactic acid was added in the medium,the lower the pH of medium was,and the lighter the mycelium was.While the production of aflatoxin B1 increased with the decrease of pH,which implied that the low pH resulted from lactic acid in medium inhibited the growth of Aspergillus flavus but not the production of aflatoxin B1.While the HB02 strain was co-cultured with Aspergillus flavus,the weight of the mycelium and the production of aflatoxin B1 were both significantly lower than those of Aspergillus flavus cultured without HB02.The results implied that the mechanism of the inhibition on the growth of fungi and the production of toxin by HB02 strain resulted from many factors involving the low pH of medium,other metabolized production and interact between microorganism.
【Fund】: 国家自然科学基金项目(30471277);; 教育部博士点基金项目(20040307043)
【CateGory Index】: S816
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