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Identification and hyperthermophilic enzyme activities of a hyperthermophilic archaeon isolated from deep-sea hydrothermal vent

WANG Shu-jun1,2,LU Zhao-xin1,Lü Ming-sheng2,LIU Hong-fei2,XU Jin-li2,YANG Lei2 (1.College of Food Science and Technology,Nanjing Agricultural University,Nanjing 210095,China;2.Jiangsu Key Laboratory of Marine Biotechnology/College of Food Engineering,Huaihai Institute of Technology,Lianyungang 222005,China)  
The identification and hyperthermophilic enzyme activities of an archaeon from a deep-sea hydrothermal vent were studied.The extremely thermophilic anaerobic archaeon strain HJ21 was irregular cocci with 1.0-1.2 μm in diameter.The optimum temperature of growth was 88 ℃.The pH range for growth was 5.0-9.0,with an optimum pH of 6.5-7.0.The NaCl concentration range for growth was 10-50 g·L-1,with an optimum concentration of 20 g·L-1.It belonged to the genus Thermococcus based on the 16S rRNA gene sequence and morphological,biochemical and physiological characteristics.The strain HJ21 produced thermostable α-amylase,pullulanase,α-glucosidase and protease.The optimal working temperatures of the α-amylase,pullulanase,α-glucosidase and protease were 95,95,100 and 100 ℃,respectively.The half-life of the α-amylase,pullulanase,α-glucosidase were 5,5 and 2 h at 90 ℃,respectively;84% of the protease remained active after incubation at 100 ℃ for 2.5 h.
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