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《Journal of Nanjing Agricultural University》 2009-02
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Isolation and proliferation effect of polysaccharide extracted from grub on the immunocytes of mice in vitro

KAN Fei,SUN Jie,TIAN Lu-yang,LI Chao,XU Ming-xu,FANG Yun,DAI Chen-sheng,ZHANG Ke-yun(College of Life Sciences,Nanjing Agricultural University,Nanjing 210095,China)  
The polysaccharides were extracted from Protaetia brevitarsis larva(grub)with homogenization,ethanol and Sevag methods.The concentration of total polysaccharides of grub was measured with phenol-sulfuric acid method.MTT assay showed that the rate of proliferation of total polysaccharides with concentration of 5 μg·mL-1 and 20 μg·mL-1 on mouse spleen cell was 48.8% and 62.2%,respectively,and the rate of proliferation of total polysaccharides with concentration of 5 μg·mL-1 and 20 μg·mL-1 on macrophages was 240% and 516%,respectively.Five polysaccharides were isolated and purified from total polysaccharides using DEAE-Sephadex A-25 and LPLC-Sephadex G-75,and the two kinds of polysaccharides were associated with the activity to improve mouse splenocytes proliferation in vitro,whose molecular weight were 42 000 and 26 000,respectively.The rate of proliferation of the two kinds of polysaccharides with concentration of 5 μg·mL-1 on spleen cell was 25.2% and 41.4%,respectively,which indicated that the two kinds of polysaccharide could significantly improve the proliferation of the immunocytes of mice in vitro.
【Fund】: 江苏省自然科学基金项目(BK2006250);; 中国博士后科学基金项目(20060390292);; 江苏省博士后基金项目(0601030B)
【CateGory Index】: R284;R285
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