Identification of grass without inflorescence collected from Korqin,Inner Mongolia
ZHANG Hongxia;JI Yanling;LU Tao;WANG Yong;LIU Baohu;WANG Zhiwei;College of Life Sciences,Nanjing Agricultural University;
On September 2011,26 infertile grasses with stromata were collected from Korqin,Inner Mongolia. A PCR-based approach was used to identify these plants. Alignment analysis conducted by DNAssist indicated sequences of these plants and Leymus chinensis were 740 bp,with only four bases different in coverage,while sequence of Psathyrostachys fragilis was 1 750 bp with distinct base difference. Phylogenetic tree based on trnT-trnL intergenic space from cpDNA region revealed that these plants clustered with Leymus chinensis with a bootstrap value of 95%. Leaf epidermis micromorphology was observed by tissue clearing method. Micromorphology of these plants was also consistent with Leymus chinensis,different from others. In combination of trnT-trnL intergenic space analysis and leaf epidermis,plants with stromata collected from Korqin were identified as Leymus chinensis Trin. The host specific fungal endophyte which develops stromata on Leymus chinensis in nature had no record. This could be another criterion to distinguish Leymus chinensis plants from other plants.