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《Journal of Nanjing Normal University(Natural Science Edition)》 2007-02
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Authentication of Crocus sativus L.and its Adulterants by rDNA ITS Sequences and Allele-Specific PCR

Mao Shanguo1.2,Luo Yuming2,Shen Jie1,Ding Xiaoyu1(1.School of Life Science,Nanjing Normal University,Nanjing 210097,China)(2.Department of Biology,Huaiyin Teachers College,Huaian 223300,China)  
The PCR products of rDNA ITS regions of Crocus sativus L.and its adulterants were sequenced and analyzed using Clustal X、Mega 3.0 software.The results showed that the full length of rDNA ITS regions of C.sativus was 650 bp,and the percentage of GC contents was 60.3%,which displayed the remarkable distinction between rDNA ITS regions of C.sativus and its adulterants.In addition,a pair of allele-specific PCR(AS-PCR) primers were designed to authenticate C.sativus and its adulterants accurately without sequencing.
【Fund】: 江苏省普通高校高新技术产业发展项目基金(JH02-124)资助项目
【CateGory Index】: S567.239
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