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《Journal of Nanjing Normal University(Natural Science Edition)》 2017-04
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Isolation and Identification of Specific Spoilage Organisms in Conditioning Crayfish Products

Zhou Tao;Song Fangxia;Huang Cunhui;Ginling College,Nanjing Normal University;  
In order to isolate and identify the spoilage organism from conditioning crayfish products under 4 temperature conditions,the spoilage bacteria were separated from the conditioning crayfish products stored under low temperature of4,while the total number of colonies is 7.05 logcfu/mL in conditioning crayfish products. Isolation and identification of spoilage bacteria were performed by traditional methods of isolation and purification,colony morphology observation,physiological and biochemical identification and 16 S rDNA sequence analysis. The results showed that,85 strains were isolated from the conditioning crayfish products samples and 5 strains of typical dominant bacteria obtained by microscopy,physiological and biochemical identification and 16 S rDNA sequencing. Respectively,Bacillus cereus,Bacillus methylotrophicus,Lysinibacillus fusiformis,and Macrococcus caseolyticus.
【Fund】: 江苏省农业科技自主创新资金项目(CX(16)1057)
【CateGory Index】: TS254.7
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