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《Journal of Nanjing Railway Medical College》 1993-02
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STUDIES ON CULTIVATION OF ROTAVIRUS IN CELL CULTURE

Meng Jihong Zhang Jianqiong Lin Ling (Department of Microbiology,Nanjing Railway Medical College,Nanjing)  
The difficulties of rotavirus(RV)propagation in cell culture are not overcome completely as yet.For this reason screening a new cell line for RV propagation was carried out.After RVs were inoculated in the cultures of Frhk-4 cells,distinct cytopathogenic effects(CPE)developed.RV antigens and nucleic acids were detected in the infected cultures by ELISA and PACE,respective- ly.RV particles were observed on the ultra—thin sections of the infected Frhk—4 cells by EM. The results indicate that Frhk—4 is a new cell line susceptible to RV and may be a useful model for study of RV infection.In addition,the effects of concanavalin A(Con A)on RV propagation were observed under different conditions.The plaque number,productive titer and CPE degree of Wa strain significantly increased when certain dosages of Con A were added into the cell culture medium to pretreat the cells in the process of growth.The plaque number,however,significantly decreased under other conditions.Thus Con A is suggested to enhance or inhibit the RV multipli- cation in vitro under different condition.Moreover,it is interesting that the electrophoretic model of the genomic RNA of Wa strain changes after being cultured on MA104 cells for several pas- sages.Three variants were cloned from the“Wa”strain with the electrophoretic model of 4:3:4: 2.The genomic RNA of the variants was composed of 11 RNA segments,but the electrophoretic patterns were different from each other and that of Wa strain.The results suggest that the muta- tions and rearrangements of the genome of Wa strain may occur in vitro.
【Fund】: 铁道部教育司青年科技基金
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