Expression of peroxisome proliferator-activated receptor γ in human gastric carcinoma cell and inhibitory effects of pioglitazone
MA Jian-xia,LIU Shun-ying (Department of Gastroenterology,Zhongda Hospital,Southeast University,Nanjing 210009,China)
Objective To study the peroxisome proliferators-activated receptor(PPAR)γ expression in human gastric cancer MKN?45 cells and the effect of PPARγ ligand on proliferation of gastric carcinoma cell line and on the changes of c-myc gene expression.Methods Reverse transcripition-polymerase chain reaction was used to demonstrate PPARγ expression in MKN?45 cell line.PPARγ agonist(pioglitazone) showed dose-dependent inhibitory effects on the proliferation of the gastric cancer cells.Pioglitazone also reduced c-myc gene expression in MKN?45 cells.Results Peroxisome proliferators activated receptor(PPAR)γ was expressed in MKN?45 cells. 0.01?μmol·L -1 pioglitazone had no effect on proliferation of MKN?45 cells. MKN?45 cells cultured by 1?μmol·L -1 pioglitazone for 48 hours showed inhibitory effect and c-myc gene demonstrated a significant decrease.Conclusion These results suggest PPARγ agonist(pioglitazone) can inhibit proliferation of gastric carcinoma cell line.And the reduction of c-myc gene expression can be one of the mechanisms of the antiproliferative effect of PPARγ activation in human gastric cancer cells.
【CateGory Index】： R735.2