The Effect of Curcumin on Nephrotoxic Serum Nephritis in Rats
BAO Hua-ying , CHEN Rong-hua, GUO Mei, FEI Li, PAN Xiao-qin, HUANG Wen-yan, WU Yuan-jun,ZHANG Ai-hua ( Center of Pediatric Nephrology, NJMU, Nanjing 210029, China)
Objective: To investigate the effect of curcumin on nephrotoxic nephritis in rats. Methods: Healthy male Sprague-Dawley rats were randomly divided into three groups, 24 animals for each group. The rats of normal control group were injected introvenously with normal saline 0. 5 ml/d for two days. The rats of nephrotoxic serum nephritis (NTN) group were injected intravenously with 0. 5 ml/d nephrotoxic serum for two days. The rats of curcumin treated group were injected introvenously with 0. 5 ml/d nephrotic serum for two days, and meanwhile received 50 mg · kg-1·d-1 of curcumin intraperitoneally every day. Animals were killed on days 3, 7, 14, 28 in batch and the samples of urien and kidney were collected. Twenty-four hours urine protein was measured by sulf-salicylic acid method and medical imaging analysis system was performed to assess the pathological damage of the kidney. Results: Treatment of NTN rats with curcumin resulted in less proteinuria ( P 0. 01), less interstitial tubular damage ( P 0. 01) and attenuation of the cell proliferation in glomerulus as compared with those in NTN animals without curcumin treatment ( P 0. 01). Conclusion: Curcumin reduces renal injury induced by anti-nephrotoxic serum.