Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Academiae Medicinae Nanjing》 2005-05
Add to Favorite Get Latest Update

The effects of genistein on the proliferation and cell cycle in human breast cancer cell MCF-7

ZHANG Guo-fu,LI Zhong*,MO Bao-qing,WEI Wei(Department of Nutrition and Food Science, School of Public Health of NJMU, Nanjing 210029, China)  
Objective:To study the effects of genistein(Gen) on the proliferation and cell cycle progression in human breast cancer cell MCF-7. Methods:The effects of Gen on the growth of MCF-7 cells were observed by MTT assay. Flow cytometry was used to analyze the cell cycle. The cyclin B and cyclin E protein expressions were observed using Western blotting method. Results:Gen inhibited the proliferation of MCF-7 cells significantly, arrested cell cycle progression at G2/M phase, and enhanced cyclin B protein expression with dose-dependent manner. No effect on cyclin E protein expression was observed. Conclusion:Growth inhibition and G2/M arrest of MCF-7 cells after treated with Gen may be related to the accumulation of cyclin B protein,rather than cyclin E protein expression.
【Fund】: 国家自然科学基金资助项目(NO.30271122)
【CateGory Index】: R737.9
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved