Anti-respiratory syncytial virus(RSV,Long strain) effect of Reduning inject on in vitro
FENG Gan-zhu,ZHOU Feng1,HUANG Mao2,YAO Kun1 (Department of Respiratory,the Second Affliated Hospital of NJMU,Nanjing 210011;1Department of Microology-Immunology,NJMU,Nanjing 210029; 2Department of Respiratory, the First Affliated Hospital of NJMU,Nanjing 210029,China)
Objective:To explore anti-reispiratory syncytial virus(RSV) effect of Chinese medicinal Reduning injection in vitro. Method:The technique of cell culture and MTT with ribavirin as positive control to observe anti-reispiratory syncytial virus effect. Results:This drug was found to be a potential inhibitor of RSV in a concentration-dependent manner with the median toxic concentration(TC50) of 3.972 g/L in Hep-2 Cell culture. The median effective concentration(EC50) was 0.428 g/L-1 when drug was added 24 h before infection. The EC50 was 1.160 g/L when drug was added 2 h after virus infection,and it was 1.189 g/L as the drug was added after it mixed with virus 24 h. The therapeutic index(TI) was 9.28,3.42 and 3.34 respectively. Conclusion:Reduning injection has the direct deactivation and blocking effect on RSV. The preventive effect is the most evident in three drug deliveries drug with the same concentration in virto.
【CateGory Index】： R285.5