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《Acta Universitatis Medicinalis Nanjing(Natural Science)》 2014-11
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Preparation of c RGD modified doxorubicin liposomals and the targeting effects on Hela cells

Chen Yu;Yang Lei;Li Xiangdong;Chen Wanying;Wang Tao;Ren Yuying;Wang Yapeng;Chen Daozhen;Central Laboratory,Wuxi Maternity and Child Health Care Hospital Affiliated to NJMU;Department of Pathology and Pathophysiology,School of Medical Science,Southeast University;Clinical Laboratory,the People's Hospital of Aheqi County;  
Objective:To prepare doxorubicin liposomals modified with PEG and c RGD,and study physicochemical properties of liposomals along with targeting effects on Hela cells. Methods:Using p H gradient method to prepare the liposomes modified with c RGD. The characteristics were observed with TEM and UV spectrophotometer. The targeting effect was determined by immunofluorescence and flow cytometry on Hela cells,and cell apoptosis was measured by MTT assay and flow cytometry. Results:The particle size of liposomes was between 100-180 nm,and encapsulation ratio detected by UV spectrophotometer was 72%. The result of immunofluorescence and flow cytometry showed targeting effect of liposomes on Hela cells,which was significantly better than that of control group. Conclusion:These doxorubicin liposomes with high encapsulation ratio and small particle size exhibited an effect of cell targeting and cell penetrating,which might be a novel approach for specific and efficient intracellular drug delivery.
【Fund】: 新疆维吾尔自治区自然科学基金资助(201318101-13)
【CateGory Index】: R944
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