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《Journal of Nanjing University of Traditional Chinese Medicine》 2010-01
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Inhibiting Effect of Phyllanthus Urinaria Alcohol Extract on Human Stomach Cancer Cell MKN28

JI Jian-mei,HU Wen-jing,YU Li-xia,QIAN Xiao-ping,LIU Bao-rui(Oncology Center of Gulou Hospital affiliated to Medical College of Nanjing University,Clinical Tumor Research Institute of Nanjing University,Nanjing,Jiangsu,210008,China)  
OBJECTIVE To carry out in vitro experiment to investigate into the effect of phyllanthus urinaria alcohol extract on the growth of human stomach cancer cell MKN28.METHOD Colony formation test was adopted to observe the effect of MKN28,MTT method was used to determine its multiplication-inhibiting rate,and flow cytometry was used to detect MKN28 cell apoptosis 48 hours after phyllanthus urinaria alcohol extract took effect.RESULT Under two different concentrations(2 mg/mL and 1 mg/mL),the extract had different degrees of inhibiting effect on MKN28 colony formation,and the higher the concentration,the better the inhibiting effect.MTT method determination showed that the extract had inhibiting effect on the multiplication of MKN28 cells and the inhibiting effect was dependent on concentration and time.The result of flow cytometry suggested that the extract could induce MKN28 apoptosis.CONCLUSION The alcohol extract of phyllanthus ruinaria can inhibit the growth of human stomach cell MKN28,and apoptosis is one of its mechanisms.
【Fund】: 国家自然科学基金(30872471);; 江苏省自然科学基金(BK20080864)
【CateGory Index】: R285.5
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