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《Journal of Nanjing University of Traditional Chinese Medicine》 2010-01
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Inhibiting Effect of Phyllanthus Urinaria Alcohol Extract on Human Stomach Cancer Cell MKN28

JI Jian-mei,HU Wen-jing,YU Li-xia,QIAN Xiao-ping,LIU Bao-rui(Oncology Center of Gulou Hospital affiliated to Medical College of Nanjing University,Clinical Tumor Research Institute of Nanjing University,Nanjing,Jiangsu,210008,China)  
OBJECTIVE To carry out in vitro experiment to investigate into the effect of phyllanthus urinaria alcohol extract on the growth of human stomach cancer cell MKN28.METHOD Colony formation test was adopted to observe the effect of MKN28,MTT method was used to determine its multiplication-inhibiting rate,and flow cytometry was used to detect MKN28 cell apoptosis 48 hours after phyllanthus urinaria alcohol extract took effect.RESULT Under two different concentrations(2 mg/mL and 1 mg/mL),the extract had different degrees of inhibiting effect on MKN28 colony formation,and the higher the concentration,the better the inhibiting effect.MTT method determination showed that the extract had inhibiting effect on the multiplication of MKN28 cells and the inhibiting effect was dependent on concentration and time.The result of flow cytometry suggested that the extract could induce MKN28 apoptosis.CONCLUSION The alcohol extract of phyllanthus ruinaria can inhibit the growth of human stomach cell MKN28,and apoptosis is one of its mechanisms.
【Fund】: 国家自然科学基金(30872471);; 江苏省自然科学基金(BK20080864)
【CateGory Index】: R285.5
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1 Huang Yuhua, Zhang Jianjun, Yan Xuesheng, Peng Shenan, Wang Boxiang (Affiliated Hospital of Hubei College of TCM Wuhan 430061);Effects or Phyllanthus Urinarial (PUL) on the Differentiation of Human Hepatocarcinoma Cell Strain Bel-7402[J];JOUNAL OF HUBEI COLLEGE OF TRDITIONAL CHINESE MEDICINE;2000-01
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1 SHI Xin-qiang,SHEN Ye-shou,WEI Zi,MA Jin-bao(Institute of Biochmistry and Microbiology,School of Life Science,Anhui Universitya;The Key Lab of Chinese Traditional Medicine Research and Development of Anhui Province,Hefei 230039,China);Inhibitory Effects of Phellinus Linteus Intracellular Polysaccharide on the Proliferation of Leukemic Cells K562[J];Carcinogenesis,Teratogenesis & Mutagenesis;2007-06
2 YUE Zhen et al(Department of Biochemistry,Binzhou Medical College,Binzhou,Shandong 256603);Study on the Seperation,Purification,Characteristics of Laminaria Polysaccharide Sulfate[J];Journal of Anhui Agricultural Sciences;2010-16
3 ZHANG Bo1,ZHENG Lan2,HUANG Yu-wen2,MO Qin2,WANG Xun2,QIAN Kai-cheng2(1.Shanghai Blood Center Graduate Student Training Base,East China Normal University,Shanghai 200051,China; 2.Shanghai Blood Center,Shanghai 200051,China);Using Real-Time PCR to Evaluate the Effect of Viral Inactivation by Methylene Blue with Visible Light[J];Chinese Journal of Virology;2009-04
4 WANG Guilong HOU Yudong SHI Lingbo Department of Stomotology,Binzhou Medical University,Binzhou 256603;Effects of smokeless tobacco extract on the rat's osteoblasts in vitro[J];Journal of Binzhou Medical University;2009-02
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7 JIANG De-xun1,XU Pu2,SHEN Ai-guo3,QIN Yong-wei3,XU Jian-rong2,KONG Xiang-ting2,SHEN hui4,YANG Jia-xin1 (1.College of Life Sciences,Nanjing Normal University,Nanjing 210097;2.Department of Biology and Food Engineering,Changshu Institute of Technology,Changshu,Jjiangsu 215500;3.School of Basic Medical Science,Nantong University,Nantong,Jiangsu 226001;4.Jiangsu Institute of Marine Fisheries,Nantong,Jiangsu 226007);A Preliminary Study of Foot and Mantle Tissues Culture from Ampullaria gigas[J];Freshwater Fisheries;2008-02
8 KONG Xiang-ting1,SHEN Song-dong1,JIANG De-xun2,JIANG Hong-xia2,XU Jian-rong2,ZHANG Jun2,HAN Xiao-lei2,XU Pu2(1.Department of Cell Biology,Soochow University,Suzhou,Jiangsu 215123;2.Department of Biology and Food Engineering,Changshu Institute of Technology,Changshu,Jiangsu 215500);A preliminary study on snout tissue culture from Pelteobagrus fulvidraco[J];Freshwater Fisheries;2009-02
9 WANG Liju*, WANG Qi, WANG Zhenshan, YU Zhihong, WANG Jinguang, XIONG Hai, YIN Qiang, WANG Yingyan, WANG Tao, TONG Shuping. *Department of Respiratory Diseases, the Dalian Friendship Hospital, Dalian, Liaoning 116001, P.R.China;Relationship between chemosensitivity in vitro and the expression of GRP78 in non-small cell lung cancer[J];Chinese Journal of Lung Cancer;2006-06
10 TIAN Yong-gang1,XU Jun2,LIU Chang2.1.The 2nd Affiliated Hospital of Harbin Medical University,Harbin 150086,China;2.The 4th Affidiated Hospital of Harbin Medical University;The biological behavioral influence of artificial pneumoperitoneum on colon carcinoma cell[J];Journal of Laparoscopic Surgery;2009-10
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2 Zhou Chunhua,Shen Xingyong,Xiao Yangfan,et al Zhejiang Chinese Medical Unversity,Hangzhou(310053);Pro.Zhou Weishun’s Experience in Treating Oesophagus Cancer with TCM[J];Journal of Zhejiang University of Traditional Chinese Medicine;2009-01
3 ZHENG Hai-yin,WU Yi-man,LIN Xin-fu,et al(Department of Integrative Medicine,FUTCM,Fuzhou,Fujian 350108,China);Detection of Apoptosis of Hepatoma Cells Induced by Salvia chinensis Benth. Polysaccharides by Flow Cytometry[J];Journal of Fujian University of Traditional Chinese Medicine;2009-03
4 Liu Yongping1,2,Ling Yang2,Hu Wenjing1,Xie Li1,Yu Lixia1,Qian Xiaoping1,Liu Baorui1 Department of Oncology,Nanjing Drum Tower Hospital,Nanjing University of Chinese Medicine,Nanjing,Jiangsu,China 210008 Clinical Oncology Laboratory,Changzhou Tumor Hospital Affiliated to Medical School of Suzhou University,Changzhou,Jiangsu,China 213002;In Vitro Interaction between CLFF and Oxaliplatin or Docetaxel in Human Gastric Cancer Cell Lines[J];China Journal of Chinese Medicine;2010-02
5 Huang Yuhua, Zhang Jianjun, Yan Xuesheng, Peng Shenan, Wang Boxiang (Affiliated Hospital of Hubei College of TCM Wuhan 430061);Effects or Phyllanthus Urinarial (PUL) on the Differentiation of Human Hepatocarcinoma Cell Strain Bel-7402[J];JOUNAL OF HUBEI COLLEGE OF TRDITIONAL CHINESE MEDICINE;2000-01
6 ZHAO Ming-Hong~ ①② ,GUO Tao,WANG Min-Wei~②,ZHAO Qing-Chun (~①The General Hospital of Shenyang Military Region, Shenyang 110016,Liaoning China) (~②The school of pharmacy, Shenyang Pharmaceutical University, Shenyang 110016,Liaoning China);Mitochondria-dependent Mechanisms Involved in HepG2 cell Apoptosis Induced by Uncarinic Acid E[J];Pharmaceutical Journal of Chinese People's Liberation Army;2006-06
7 LI Feng, ZHANG Wei, WANG Yuqin ,et al (Department of pharmacology, Nantong University, Nantong 226001);Experimental study on anti-tumor effect of Fufang Tiangjian Wan,a Chinese drug on mice with transplanted tumors[J];Journal of Nantong University(Medical Sciences);2006-02
8 WANG An yu 1,ZHU Xiao dong 1,CHEN Long 1,WANG Shao feng 2,HUANG Ren bin 3 1.Department of Radiotherapy,Cancer Hospital of Guangxi Medical University,Nanning 530021,P.R.China 2.Department of Radiotherapy,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,P.R.China 3.College of Pharmacy,Guangxi Medical University,Nanning 530021,P.R.China;Inhibiting effect of Fuzheng Anticancer capsule No.1 on the growth of experimental tumor cell lines both in vivo and in vitro[J];Journal of Qilu Oncology;2004-01
9 ZHAO Xiao-qin Affiliated Hospital of Hubei Institute for Nationalities;Dr Zhao Changji's Experience in Treating Tumors by Medicinal Herbs[J];Shanghai Journal of Traditional Chinese Medicine;2006-08
10 HU Jing,QIAN Xiao-ping,LIU Bao-rui,ZHU Li-jing,HU Wen-jing,SUN Jing,WANG Li-feng,YU Li-xiaCancer Center of Nanjing University Medical College Affiliated Drum Tower Hospital,Nanjing 210008,China.;Inhibitory effect of paridis on angiogenesis in vitro[J];Journal of Modern Oncology;2008-08
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