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《Journal of Agricultural Science and Technology》 2012-06
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Studies on Drought Tolerance of IPT Transgenic Rice

MAIMAITI Yu-shan-jiang1,XIONG Ye-hui1,MIJITI Mai-he-mu-ti-jiang1,SEHROON Khan1, ANDROW Cal2,ZENG Hong-mei1,QIU De-wen1*(1.Key Laboratory for Biological Control of Agricultural,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100081,China;2.Gene Research Lab.of Plant Breeding and Bio-chemistry Division,International Rice Research Institute,Metro Manila,DAPO Box 7777,Philippines)  
Drought resistance has become a hot issue in rice scientific research.By Agrobacterium tumefaciens-mediated,IPT gene was transformed to rice IR64 variety.254 regenerated were obtained,and PCR and Southern blotting analyzsis were carried on them to confirm integration of target gene in the transgenic plant.Two transgenic single copy events and one wild type were treated with drought stress at flowering stage.Under the same drought stress condition,the cytokinins and chlorophyll contents of transgenic plants were obviously higher than that of the wild type.While the leaf temperature was slightly lower than that of the wild type.The stem and leaf senescence of transgenic plant was delayed.These result shows that IPT gene has positive significance on studying drought tolerance breeding and its mechanism.
【Fund】: 国家转基因生物新品种培育重大专项(2009ZX08001-018B)资助
【CateGory Index】: S511
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