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Preliminary Investigation of Relationship Between Sperm Apoptosis and Male Infertility

Guang Rong WANG, Zeng Di ZHOU, Zheng Min GE, Ming Jun ZHAO (Family Planning Research Institute of Yunnan, Kunming,Yunan 650021,China)  
Objectives: To evaluate the relationship between sperm apoptosis and male infertility. Methods: Percentage of apoptotic sperm (PAS)in spermatozoa of fertile and infertile men were tested by flow cytometry (FCM). Results: Sperm apoptosis had happened in all different people. PAS in fertile and infertile group was (4.28±1.66)% and (18.67±8.55)% respectively,and difference was significant between two groups ( P 0.01).There was negative correlation between PAS and semen volume, sperm density, percentage of forward motility, percentage of normal morphology ( P 0.01). Conclusions : There was very close relationship between sperm apoptosis and male infertility. FCM used to test sperm apoptosis is rapid, accurate, objective and reliable to analyse sperm functions and male fertility.
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