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《National Journal of Andrology》 2006-08
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Expression Characteristics of Mouse Catsper——the Structural Protein of Sperm Hyperactivation

YANG Yuan-yuan,WANG Gen-linAnimal Science and Technology College,Nanjing Agricultural University,Nanjing,Jiangsu 210095,China  
Objective: To study the expression specificity in tissues of the Catsper and its expression rules,which is a specific cation channels in sperm and plays an important role in sperm hyperactivation. Methods: The expression of Catsper in eight different tissues was examined by RT-PCR and immunohischemistry,the location of Catsper in sperm determined by immunofluorescence and the expression changes of Catsper analyzed in mice of different age groups.Results: Catsper was expressed specifically in the testis and epididymis,and was mainly located in the principal piece of the sperm tail.Catsper gene expression was developmentally regulated with a direct correlation between Catsper expression and mouse sexual maturation.Catsper gene experssion was first detected at 3 weeks of age and increased significantly till 6 weeks of age and then gradually to a stationary phase.Conclusion: Catsper expresses in the testis and epididymis,and its growth period and expression amount are closely related with the developmental stages of the mouse.
【Fund】: 国家自然科学基金(30471243)
【CateGory Index】: Q492
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