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《National Journal of Andrology》 2013-04
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Establishing a mouse model of Sertoli-cell-only syndrome by administration of busulfan

LI Jing-ping1,GUO Wen-bin2,HE Jin-can2,YU Qing-feng2, WU Xiao-qiang2,MAO Xiang-ming2,ZHANG Feng-bin1 1.Department of Reproductive Endocrinology,Women's Hospital,Zhejiang University School of Medicine,Hangzhou,Zhejiang 310006,China;2.Department of Urology,Nanfang Hospital,Southern Medical University,Guangzhou,Guangdong 510515,China  
Objective: To establish a stable and reliable model of Sertoli-cell-only syndrome in mice.Methods: We randomly divided 60 NIH mice into two groups of equal number to receive intraperitoneal injection of busulfan(30 mg/kg) and 30 or 60 minutes of testis cooling.At 2,4 and 8 weeks after treatment,we recorded the survival rate of the mice,weight of the testis and Johnsen scores,and conducted quantitative analysis on the degrees of spermatogenetic failure.Results: There were no significant differences in the baseline body weight and survival rate between the intervention and control groups(P0.05).At 4 and 8 weeks,the testis weight and Johnsen score were significantly lower in the intervention group than in the control([0.04±0.01] g and [0.05±0.01] g vs [0.09±0.03] g and [0.11±0.02] g,P0.05;3.86±0.50 and 2.70±0.67 vs 9.60±0.25 and 9.76±0.43,P0.01).At 2,4 and 8 weeks,the testis weights were(0.07±0.02) g,(0.06±0.01) g and(0.09±0.01) g,respectively,in the 30-min cooling group and(0.05±0.01) g,(0.04±0.02) g and(0.04±0.02) g in the 60-min cooling group,significantly lower than in the control side at the same time points([0.11±0.01] g,[0.11±0.01] g and [0.12±0.00] g)(P0.05),and the Johnsen scores were 4.70±0.67,2.70±0.84 and 6.10±1.14 in the 30-min and 1.67±0.58,1.20±0.45 and 1.00±0.00 in the 60-min cooling group,remarkably lower than in the control side(9.60±3.23,9.60±0.55 and 9.70±0.45)(P0.01).Histopathological examination of the cooled testes revealed considerable atrophy of seminal tubules,necrosis of seminiferous epithelia and peritubular fibrosis.Conclusion: Administration of busulfan has no obvious influence on the survival of mice,and is a reliable method for constructing a mouse model of Sertoli-cell-only syndrome.
【Fund】: 国家自然科学基金(81270743);; 广东省科技项目(2010B031600092)~~
【CateGory Index】: R698.2;R-332
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