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《Acta Scientiarum Naturalium Universitatis Neimongol》 2002-06
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A Comparison of Four Pyorgallol Autoxidation Methodson Determining Superoxide Dismutase Activity

ZHANG Hong,TAN Zhujun(College of life Science,NeiMongol University,Hohhot 010021,PRC)  
There is no distinction in determining SOD activity between pyorgallol autoxidation(420nm) and pyorgallol autoxidation/dithiothreitol(DTT) method.The conversion coefficient is 1.03;The results are different in determining SOD activity with the method of improved pyorgallol autoxidation (325nm) and trifling pyorgallol autoxidation/vitaminC(Vc) as compared with pyorgallol autoxidation(420nm).And its conversion coefficient is 2.51 and 5.51 respectively.They can be conversed with conversion coefficients in different methods of determining SOD activity. The tests also show there are no effects in determining SOD activity using Vc and DTT. The improved pyorgallol autoxidation(325nm) and trifling pyorgallol autoxidation/Vc can be used in determining lower SOD activity sample due to their high sensitivity.
【CateGory Index】: Q554
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