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《Journal of Inner Mongolia University》 2010-02
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Isolation,Culture and Identification the 11 Endophytic Fungus from Oxytropis glabra DC.

HUO Hong-yan,LU Ping,NIU Yan-fang,Lü Gui-Fen,LI Song,GAO Jing,QIAN Ya-guang(College of Life Science and Technology,Inner Mongolia Normal University,Hohhot 010022,China)  
Leaves and stems were cut from individual plants of Oxytropis glabra DC.,and used as materials to isolate and culture the endophytic fungus in vitro.The microbiological morphology of colony and the conidiophore were investigated.The 5.8SrDNA/ITS of the in vitro cultured endophytic fungi were sequenced,and then the sequence data of the endophytic fungi were submitted to GenBank and analysed by homologous blast.The phylogenetic tree was constructed by MegAlign of DNASTAR software package.The molecular biological and microbiological characters were investigated in order to predict the classification of the endophytic fungi.The results indicated that the morphologic characteristics of the colony and conidiophore of this 11 endophytic fungi were the same as that of Embellisia sp.L12.The sequences of ITS1 and 5.8SrDNA of endophytic fungus from Oxytropis glabra DC.were totally the same.Compared to the sequence of Embellisia sp.L12,there was only one viriation site which was in the 5.8SrDNA region in each sequence.In addition,two variation sites were shown in the ITS2 region.It was presumed that the endophytic fungi had a close relationship to Embellisia,but the classification of the endophytic fungi need further research.
【Fund】: 国家自然科学基金资助项目(30860049)
【CateGory Index】: Q93-3
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