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《Animal Husbandry and Feed Science》 2009-10
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Cloning and Sequence Analysis of omp31 Genes of Brucella Strain Isolated from Inner Mongolia

LU Jing1,GUAN Ping-yuan1,Wurihan2,Temuerbagen2,MA Li-feng2,Juhua2 (1.College of Animal Science and Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China;2.Central of Animal Disease Prevention and Control of Inner Mongolia,Hohhot 010010,China)  
Sequence analysis was conducted on genes of omp31 from 3 different Brucella strain isolated from Inner Mongolia.A pair of primers were designed for amplification of Brucella omp31 genes according to the reported of Brucella omp31 genes complete sequence.omp31 genes were amplified from 3 different Brucella strains by polymerase chain reaction (PCR),and the PCR products were cloned into pMD19-T vector and sequences were analyzed.The results showed nucleotide sequences in homology among 3 different Brucella isolates omp31 genes were 100.0 %.Nucleotide sequence in homology was 100.0 % between the three isolates genes and Brucella suis 1330,Brucella ceti B14/94 and Brucella neotomae 5K33,its homology was 99.0 % compared with omp31 gene of Brucella canis RM6/66,Brucella melitensis183 and Brucella melitensis 16M,but the homology between the obtained and Brucella ovis ATCC25840 was the lowest,only 98.8 %.
【CateGory Index】: S852.61
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