Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Henan Agricultural University》 2005-04
Add to Favorite Get Latest Update

Application of Colloidal Gold Immunochromatographicassay in Detection of Hog Choleras Attenuated and Virulent Strain Virus Antibody

LIU Chang,ZHENG Ming,PAN Chun-mei(Deparment of Bioengineering,Zhengzhou College of Animal Hhubandry and Engineering,Zhengzhou 450011,China)  
To obtain a large amount of E2 protein in vitro,the Hog cholera lapinised virus(HCLV) E2 gene was cloned into prokaryotic expression plasmids pET30a(+).Then the recombinant expression plasmid was transformed into BL21(DE3) to express.The gene-expressed E2 protein was purified and used as attenuated HCV antigen.The PK15 cells infected by HCV titration of virulent strain were cultivated for 48 hours.Then the PK15 cells were broken into pieces and a sucrose density gradient centrifugation was performed.The proteins between 30%~35% gradient were purified,serving as virulent HCV antigen.The HCV antigens were labeled by colloidal gold.Based on the sandwich ELISA technique,immunochromatographicassay test paper for detecting HCV is firstly developed in China.The method is of discriminatory feature in serum diagnostic test for developing classical swine fever virus(CSFV) marker vaccine.It is also expected to be useful for monitoring CSFV prevalence,evaluating immunization efficacy and establishing immunization procedure.The method can also discriminate between virulent and attenuated strain,which is simpler in operation,more sensitive,and specific than other methods and fit for clinical diagnosis of HCV.
【Fund】: 河南省自然科学基金资助项目(0211031300)
【CateGory Index】: S854.4
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved