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《Journal of Henan Agricultural University》 2007-01
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Study on Indentification of Shigella and S.Pullorum by Random Amplified Polymorphic DNA Technology

YANG Xia,CHEN Lu,XU Lan-ju,LIU Hong-ying,WANG Chuan-qing(Engineering College of Animal Husbandry and Veterinary Science,Henan Agricultural University,Zhengzhou 450002,China)  
The genetic diversity among strains of chicken Shigella boydii,Shigella dysenteriae and S.Pullorum was studied by random amplified polymorphic DNA(RAPD) with 6 random primers.The results showed that four efficient primers were selected from six 10 bp random primers.Fifty-nine RAPD bands were identified and 7 of them were common in all three strains tested and 52 of them were polymorphic between any two strains.The polymorphic bands accounted for 88.1% of the total amplified bands.The primer P6 could be used to identify different species.The result showed that the common rate of the amplified bands between Shigella boydii and Shigella dysenteriae was 67%,but the amplified bands produced by Shigella and S.Pullorum was much diferent from each other when primer P6 was used.
【Fund】: 国家自然科学基金资助项目(30671555)
【CateGory Index】: S852.61
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