Study on DNA extraction of AFLP reaction system for Paulownia plants
ZHANG Yan-zhao,CAO Xi-bing,ZHAI Xiao-qiao,FAN Guo-qiang (Institue of Paulownia,Henan Agricultural University,Zhengzhou 450002,China)
The purity and yield of DNA extracted with 3 different kinds of methods from Paulownia tomentosa ×Paulownia fortunei leaves were investigated with ultraviolet-spectrophotometer and molecular technique.The results indicated that the yield of DNA extracted with DNA kit method,CTAB-Ⅰmethod and CTAB-Ⅱmethod were 0.250,0.284 and 0.312 mg·g-1 respectively,and the fragment of DNA extracted with CTAB methods was longer than that extracted with DNA kit.The DNA extracted with CTAB-Ⅱcould be completely digested with EcoRI,MspⅠ and HpaⅡ.And its band patterns of AFLP-PCR products were clear,stable and reproducible with gel electrophoresis.So CTAB-Ⅱ was considered to be the best DNA extraction method for Paulownia plants.
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