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《Journal of Henan Agricultural University》 2010-05
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Biotype identifications and phylogenic analysis of overwintering Bemisia tabaci in protecting fields of Henan Province

JI Kun,BAI Run-e,TANG Qing-bo,JIANG Jin-wei,ZHANG Da-shan,YAN Feng-ming(Institute of Chemical Ecology,Henan Agricultural University,Zhengzhou 450002,China)  
In this study,the mitochondria cytochrome oxidase I(mtDNA COI) gene of Bemisia tabaci was used to identify the biotypes of 5 representatively geographical locations in Henan,China.Furthermore,the phylogenic relationship with other geographical biotypes of B.tabaci was deduced.Results showed that 2 biotypes of B.tabaci were found in Zhengzhou overwintering fields with B biotype in calla lily(Zantedeschia aethiopica) and Q biotype in tomato.While the biotypes of B.tabaci in Anyang,Xinxiang,Sanmenxia and Xinyang were Q biotype with an identical nucleotide sequence of COI.Results of phylogenic analysis showed the variances of COI between Zhengzhou B and others B biotypes of different locations were not significant.Q biotypes COI sequences of Anyang,Xinxiang,Sanmenxia and Xinyang were clustered in the same branch as Florida Q2(FJ188524) in phylogenic tree,while the sequence of Zhengzhou-2 was clustered to another branch alone with a phylogenic location between Florida Q1(FJ188559)and Florida Q3(FJ188570).Results above suggested that the original invasion locations of Q biotypes of B.tabaci were different between Zhengzhou and other areas of Henan Province.
【Fund】: 国家自然科学基金项目(30571219);; 国家转基因新品种培育重大专项项目(2009ZX08012-007B 2008ZX08011-002)
【CateGory Index】: S433.3
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