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《Journal of Henan Agricultural University》 2012-06
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Study of storage methods and in vitro germination of Lagerstroemia indica pollen

QIN Meng,LI Yan,TANG Li-dan,LEI Feng-li,WANG Xian(College of Forestry,Henan Agricultural University,Zhengzhou 450002,China)  
Using the mixed pollen of four common breeds Lagerstroemia indica,L.indica 'Fen Jiaorong',L.indica 'Honghong Huohuo',L.indica 'Jinwei' and L.indica 'Zhizhi Zi' as materials,and adopting the orthogonal test methods optimizing experments were conducted on the three factors affecting pollen viability(sucrose,boric acid,and CaCl2) to determine the best combination of pollen germination according to the result of orthogonal experiment.And on that basis,pollen storage tolerance was tested under 18 storage conditions.The results were as follows: the optimal culture medium was 200 g·L-1 sucrose+150 mg· L-1 boric+20 mg· L-1 CaCl2;sucrose showed the most obvious effect on pollen germination rate of Lagerstroemia indica followed by boric and CaCl2;there are differences in pollen storage tolerance with different storage conditions.During the 18 kinds of methods,the one dealing with allochroic silica gel under the normal temperature of 28 ℃ could keep the longest pollen viability;another one dealing with CK,shading,drying under the refrigerated condition of 4 ℃ would keep a shorter time,but could get a higher pollen germination rate during storage.
【Fund】: 河南省教育厅自然科学研究项目(2008A220001)
【CateGory Index】: S685.99
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