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《Journal of Nantong University(Medical Sciences)》 2015-03
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Effects of sevoflurane postconditioning on expression of TLR4 in rats with acute lung injury induced by lipopolysaccharide

WEI Lei;YANG Fen;ZHANG Xia;ZHAO Jianhua;Department of Anesthesiology, Suzhou Municipal Hospital,Jiangsu Province;  
Objective: To observe the effects of sevoflurane postconditioning on the expression of Toll-like receptor 4(TLR4) in rats with acute lung injury induced by lipopolysaccharide(LPS). Methods: Twenty-four adult male Sprague-Dawley rats, weighing 230-280 g, were randomly assigned into 4 groups(n=6 each): normal saline group(group C), group(NS+sevoflurane)(group S), group LPS(group L), group(LPS+sevoflurane)(group LS). Rats received intratracheal instillation of normal saline1 m L/kg or LPS 5 mg/kg respectively. Group S and group LS, 4 h after LPS intratracheal instillation(i.e.after the occurrence of acute lung injury), rats received 2.4% sevoflurane inhalation for 1 h respectively. The rats was sacrificed at 6 h after administration of LPS or NS, and lungs were removed for microscopic examination and for determination of TLR4 m RNA and protein expression in lung tissues, by using reverse transcriptase polymerase chain reaction(RT-PCR) and immunohistochemistry in all groups. Concentration of tumor necrosis factor-α(TNF-α), interleukin l beta(IL-1β) in bronchoalveolar lavage fluid were measured by enzyme- linked immunosorbent assay( ELISA). Results : Compared with group C, lung tissue damage, the expression of TLR4 m RNA and protein and concentration of TNF-α, IL-1β in bronchoalveolar lavage fluid were obviously increased in group L and group LS(P0.01). Compared with group L, the parameters mentioned above were significantly decreased in group LS(P0.01 or P0.05). Conclusion: Sevoflurane postconditioning could reduce LPS-induced acute lung injury in rats through inhibiting the up-regulation of TLR4 expression in lung tissues and reducing the inflammatory response.
【Fund】: 南京医科大学科技发展基金面上项目(2014NJMU104)
【CateGory Index】: R614
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Chinese Journal Full-text Database 2 Hits
1 ZHAO Shuangping,WU Jiao,GUO Qulian,ZHANG Zhong,YE Zhi(Intensive Care Unit,Xiangya Hospital,Central South University,Changsha 410008,China);Effect of different concentrations of sevoflurane pretreatment on acute lung injury induced by endotoxin in rats[J];Journal of Central South University(Medical Science);2010-09
2 YANG Fen;ZHAO Jian-hua;Department of Anesthesia,Suzhou Municipal Hospital;;Effects of postconditioning with sevoflurane on iNOS/NO pathway in rats with acute lung injury induced by lipopolysaccharide[J];Journal of Jiangsu University(Medicine Edition);2013-06
Chinese Journal Full-text Database 10 Hits
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2 CHEN Xiao-xuan;DAI Jian-ping;WAN Qian-ying;ZHU Dan-xia;WANG Ge-fei;LI Kang-sheng;Department of Microbiology and Immunology, Shantou University Medical College;;The effects and mechanisms of oxymatrine on anti-influenza A virus activity[J];Carcinogenesis,Teratogenesis & Mutagenesis;2014-03
3 Zhang Hongqin;Wang Diping;Li Ping;Xiao Wei;Xu Lu;Department of Anesthesiology,the Third People′s Hospital of Chongqing;Department of Anesthesiology,the First People′s Hospital of Chongqing;;The effect of sevoflurane pretreatment or aftertreatment on intrapulmonary shunt for patients with one lung ventilation during thoracic surgical procedures[J];Chongqing Medicine;2014-30
4 WANG Dong-xin;HOU Wen-jie;DIAO Shi-qi;KAN Hong-li;Department of Anesthesiology,Jilin Province Tumor Hospital;;Effect of sevoflurane preconditioning on inflammatory cytokines and oxygen radicals during one lung ventilation in patients with lung cancer[J];Journal of Clinical Anesthesiology;2013-09
5 ZHAO Li-li;RONG Hao;SUN Fang-yun;Lab for Basic Research of Life Science,Tibet University for Nationalities;;Pathogenesis research on lipopolysaccharide-induced acute lung injury[J];Practical Pharmacy and Clinical Remedies;2015-04
6 SUN Yan-hong,CUI Yong,WANG Jun-ke,PEI Ling(Department of Anesthesiology,First Affiliated Hospital of China Medical University,Shenyang 110001,China);Effect of sevoflurane on pulmonary ICAM-1 and TNF-α after acute lung injury induced by endotoxin in rats[J];Chinese Journal of New Drugs;2011-22
7 XIONG Zhangrong1,QI Jun2,LIU Xingming3,ZHANG Zhi2,MU Lin1,WEI Chuang1,TANG Yanxian1(1.Department of Anesthesiology;2.Department of Thoracic Surgery;3.Center Laboratory, Tumour Institute of Chongqing);Influences of sevoflurane inhalation in advance on pulmonary function and oxidative stress responses during one-lung ventilation[J];Journal of Chongqing Medical University;2013-03
8 Shu-Ye Song,Bing Zhou,Shuang-Mei Yang,Guo-Ze Liu,Jian-Min Tian,Xiu-Qin Yue~* Department of Anesthesiology,Xinxiang Medical College First Affiliated Hospital,Weihui 453100,Henan,China;Preventive effects of sevoflurane treatment on lung inflammation in rats[J];亚太热带医药杂志(英文版);2013-01
9 ;Basis and clinical research of acute lung injury /acute respiratory distress syndrome[J];Chinese Journal of Lung Diseases(Electronic Edition);2013-04
10 QU Yu-lian;HE Qing-quan;GONG Yan;YANG Hai-feng;CHEN Jian-shu;CHEN Hong-xia;Department of Anesthesiology,the 2nd Hospital of Wenling;Clinical Laboratory,the 2nd Hospital of Wenling;;Effects of dexmedeomidine on oxidative stress in patients received one-lung ventilation in video-assisted thoracoscopic surgery[J];China Journal of Endoscopy;2013-11
【Secondary Citations】
Chinese Journal Full-text Database 6 Hits
1 CHANG Jia-song,WEI Kai-feng,XU De-guo(Nanjing University of Chinese Medicine,Nanjing 210029,Jiangsu,China);Effect of Shengjiang Powder on Expression of ICAM-1 of Lung Tissue Cells in Acute Lung Injury Rats[J];Journal of Liaoning University of Traditional Chinese Medicine;2008-01
2 GUO Zhengming1,CHEN Yi2,LIU Huijun2,XIAO Yuequn1,SONG Haipeng1,WANG Zhaozhao1,QU Xiaolin1,LUO Ziqiang2(1.Department of Basic Science,Hunan Traditional Chinese Medicine College,Zhuzhou Hunan 412012;2.Department of Physiology,Xiangya School of Medicine,Central South University,Changsha 410078,China);Ginkgo biloba extract (Ginaton) attenuates acute lung injury induced by lipopolysaccharide in mice[J];International Journal of Pathology and Clinical Medicine;2009-04
3 SUN Yan-hong (?) Yong WANG Jun-ke Department of Anes(?)siology,Frist Affi(?) (?) of (?) Medical University,Shenyang 110001,China;Effects of sevoflurane on endotoxin-induced oxidative stress response of the lungs in rats[J];Chinese Journal of Anesthesiology;2006-08
4 SUN Yan hong,ZHANG Qian,WANG Jun ke,CUI Yong Department of Anesthesiology,First Affiliated Hospital of China Medical University,Shenyang 110001,China;Effects of sevoflurane on membrane permeability of alveolar capillaries in rats with acute lung injury caused by endotoxin[J];Chinese Journal of Surgery;2004-16
5 YANG Fen,FANG Zhi-yuan(Jiangsu Province Key Laboratory of Anesthesiology,Xuzhou Medical College,Xuzhou Jiangsu 221002,China);Effects of sevoflurane postconditioning on lungs of rats with lipopolysaccharide-induced acute lung injury[J];Journal of Jiangsu University(Medicine Edition);2010-03
6 GU Da-yong,LIANG Bing,TANG Xu-dong,et al.Science Laboratory,Number 401 Hospital of PLA,Shandong Qingdao 266071,China. Clinical Experimental Institute,Chendu Army General Hospital of PLA,Sichuan Chendu 610083,China;A Research on Expression of ICAM-1 and Adhesion to PMN of PMVEC Directly-Induced by LPS[J];Journal of Chinese Microcirculation;2006-01
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