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《Journal of Nantong University(Medical Sciences)》 2015-03
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Effects of sevoflurane postconditioning on expression of TLR4 in rats with acute lung injury induced by lipopolysaccharide

WEI Lei;YANG Fen;ZHANG Xia;ZHAO Jianhua;Department of Anesthesiology, Suzhou Municipal Hospital,Jiangsu Province;  
Objective: To observe the effects of sevoflurane postconditioning on the expression of Toll-like receptor 4(TLR4) in rats with acute lung injury induced by lipopolysaccharide(LPS). Methods: Twenty-four adult male Sprague-Dawley rats, weighing 230-280 g, were randomly assigned into 4 groups(n=6 each): normal saline group(group C), group(NS+sevoflurane)(group S), group LPS(group L), group(LPS+sevoflurane)(group LS). Rats received intratracheal instillation of normal saline1 m L/kg or LPS 5 mg/kg respectively. Group S and group LS, 4 h after LPS intratracheal instillation(i.e.after the occurrence of acute lung injury), rats received 2.4% sevoflurane inhalation for 1 h respectively. The rats was sacrificed at 6 h after administration of LPS or NS, and lungs were removed for microscopic examination and for determination of TLR4 m RNA and protein expression in lung tissues, by using reverse transcriptase polymerase chain reaction(RT-PCR) and immunohistochemistry in all groups. Concentration of tumor necrosis factor-α(TNF-α), interleukin l beta(IL-1β) in bronchoalveolar lavage fluid were measured by enzyme- linked immunosorbent assay( ELISA). Results : Compared with group C, lung tissue damage, the expression of TLR4 m RNA and protein and concentration of TNF-α, IL-1β in bronchoalveolar lavage fluid were obviously increased in group L and group LS(P0.01). Compared with group L, the parameters mentioned above were significantly decreased in group LS(P0.01 or P0.05). Conclusion: Sevoflurane postconditioning could reduce LPS-induced acute lung injury in rats through inhibiting the up-regulation of TLR4 expression in lung tissues and reducing the inflammatory response.
【Fund】: 南京医科大学科技发展基金面上项目(2014NJMU104)
【CateGory Index】: R614
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