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《Journal of Ningxia University(Natural Science Edition)》 2006-03
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Phylogenetic Relationship of Some Species of Libellulinae Inferred from Sequences of Mitochondrial Cytochrome b Gene (Odonata:Libellulidae)

Zhang Dazhi1,Yang Guijun1,Zheng Zhemin2(1.School of Life Science,Ningxia University,Yinchuan 750021,China;2.School of Life Science,Shaanxi Normal University,Xi’an 710062,China)  
Partial nucleotide sequences of mitochondrial DNA cytochromebgene from 7 species of 3 genera of Libellulinae in China were amplified by PCR and directly sequenced by silver-staining sequencing technique in this paper.The sequences were parts of Cytbgene of mtDNA(576 base pairs in length),and in the obtained sequences,the average of A+T was about 70.2%.From every amino acid codon,the average of A+T in the third sit was higher(85.4%) than the other two sits.The sequence data revealed considerable variation in 180 nucleotide sites(about 31.25%) among the analyzed individuals from 3 genera,Orthetrum,LyriothemisandLibellula.We obtained homologous nuclear sequence ofMnais maclachlani(damselfly) for outgroup.The phylogenetic trees were constructed with neighbor-joining method,maximum parsimony method and Bayesian inference.The confidence of nodes in the trees was evaluated by 1 000 replicates bootstrap.The phylogenetic relationship of mitochondrial DNA suggested that Orthetrumwas more evolutional thanLyriothemisandLibellula.The phylogenetic relationships of the 3 genera was Libellula→Lyriothemis→Orthetrum.
【Fund】: 宁夏高等学校科研基金资助项目;; 宁夏大学自然科学基金资助项目(NS0511)
【CateGory Index】: Q961;Q969
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