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《Chinese Journal of Andrology》 2006-01
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The effect of testicular local heating on spermatogenic cell apoptosis in immature rat

Cui Haiyan, Qin Danian, Zhang Jiqiang Department of Physiology, Medical College of Shantou University, Shantou 515031,China Huang Guang Department of Paediatrics, the First Affiliated Hospital, Medical College of Shantou University  
Objective To study the effect of testicular local heating on spermatogenic cell apoptosis in immature rat. Methods Forty-eight male SD immature rats were randomly divided into heat treatment group(43℃,15min) and control group (22℃,15min).Each group was further divided into four sub-groups respectively according to the time 2 hours, 6 hours, 24 hours and 14 days after testicular local treatment.The spermatogenic cell apoptosis in all sub-groups was examined by means of electron microscopy and terminal deoxynucleotidyl transferase-mediated dUDP-nick end labeling(TUNEL) method. Results In the groups of heat treatment except for 2h-group, spermatogenic cell apoptosis was observed by electron microscopy. The percentage of positive TUNEL cells in the 6h heat treatment group was higher than that in the control group (P0.05), as well, but there was no difference in the percentage of positive TUNEL cells between the 2h heat treatment group and the control group, the percentage of positive TUNEL cells in the 24h and 14d heat treated groups was significantly higher than that in the control groups (P0.01). Conclusion Testicular local heating can increase the spermatogenic cell apoptosis in immature rat.
【Fund】: 广东省自然科学基金项目(0222720);; 汕头大学研究与发展基金项目(L00003)资助
【CateGory Index】: R33
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