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《Chinese Journal of Andrology》 2006-08
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The experimental study on the treatment of sub-acutely aging male rats by Icariin and testosterone

Zhang Zhenbao, Tian Shengping, Yang Jingqiu, Cai Congyue Department of Urology, Second Affiliated Hospital, Shantou University Medical College, Shantou 515041,China  
Objective To observe the effect of Icariin and T on the activity of serum SOD, the concentrations of T and E2, expression of P16 protein and cell apoptosis in testicle tissue for sub-acutely aging rats. Method 40 SD adult male rats were divided into control group, model group, Icariin group, Testosterone Undecanoate (TU) group. The SOD activity, the level of testosterone and E2 of each group were detected. HE stain was used to observe the morphological changes in the testicle tissue. S-P method was used to observe the expression of P16 protein in the testicle tissue. TUNEL method was used to detect the apoptosis of the testicle germ cells. Results SOD activity and T level decreased significantly (P0.01, P0.01), and E2 level changed insignificantly (P0.05) comparing to control group. Anaplasia was seen in the testicle tissue. The positive index of the expression of P16 protein and the apoptosis index of the testicle germ cells increased significantly comparing to the control group (P0.01). Compared to the model group, the Icariin group and the TU group both showed significantly changes such as SOD activity and T level increasing and the decreasing apoptosis index of germ cells. Anaplasia had been remarkably improved in the testicle tissue. The positive index of the protein expression of P16 gene in Icariin group changed significantly comparing to the model group (P0.01), whereas those of the TU group changed insignificantly. Conclusion Compared to the T, Icariin could not only increase the SOD activity and androgen level for sub-acutely aging rats, decease the apoptosis of germ cells, improve the anaplasia of the testicle tissue, but could also delay the senescence process of the genital gland by inhibiting the protein expression of the aging P16 gene of the germ cells.
【Key Words】： Icariin testosterone sub-acute aging rats
【CateGory Index】： R285;R96
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【References】
Chinese Journal Full-text Database 2 Hits
1 ZHANG Na~(1,2),LI Ya-li~3,NIU Si-yun~4,GAO Fu-lu~(1), CHEN Xue~1,GUO Li-na~2,ZHAO Su-ying~2(1.Department of Histology and Embryology Hebei Medical University,Shijiazhuang 050017,China;2.Department of Reproductive Medicine,the Forth Hospital of Hebei Medical University,Shijiazhuang 050011,China;3.Center of Healthy Birth,Excellent Growth and Education the People Hospital of Hebei Province,Shijiazhuan 050071,China;4.Institute of Basic Medical Sciences,the Hospital of Chengde Medical College, Chengde 067000,China);STUDY ON ANTI-AGING IN THE OVARY OF THE AGING MODEL RAT BY TRADITIONAL CHINESE MEDICINE HESHOUWUYIN[J];Acta Anatomica Sinica;2008-02
2 LI Li,WANG Xue-mei(Department of Integrated Chinese and Western Medicine,First Hospital,Peking University,Beijing 100034,China);Progress of pharmacological research on icariin[J];China Journal of Chinese Materia Medica;2008-23
【Citations】
Chinese Journal Full-text Database 7 Hits
1 SHAO Ying-Hong, MU Yi-Ming , LI Ming, LI Jiang-Yuan (Department of Endocrinology, General Hospital of PLA, Beijing 100853, China);Effects of aging on testicular steroidogenic function in rats[J];Academic Journal of Second Military Medical University;2004-05
2 Cui Yugui,Chen Ziqing,Sha Jiahao Key Laboratory,the First Affiliated Hospital of Nanjing Medical University,Nanjing,Jiangsu 210029,China(Cui YG,Chen ZQ);Key Reproductive Laboratory of Jiangsu Province,Nanjing,Jiangsu 210029,China(Sha JH);Effects of Reproductive Hormones on Spermatogenesis[J];Acto andrologica Sinica;2004-06
3 She Bairong, Qin Danian, Wang Sheng, et al School of Medicine, Shantou University, Shantou 515041, China;Effects of flavonoids from herba epimedii on the reproductive system in male rats[J];Chinese Journal of Andrology;2003-05
4 MA Hui-Ping, JIA Zheng-Ping, BAI Meng-Hai, GE Xin, HE Xiao-Ying, CHEN Ke-Ming (Dept of Pharmacology, Lanzhou General Hospital, Lanzhou 730050);Influence of total flavonoids of herba epimedii on the biochemical index of osteoporosis in rats[J];Chinese Pharmacological Bulletin;2003-02
5 LIU Wu-Jiang, XIN Zhong-Cheng, FU Jie, XIN Hua, YUAN Yi-Ming,TIAN Long, YANG Xin-Yu, GUO Ying-Lu (Dept of Urology, the First Hospital of Peking University, Beijing 100034);Effect of Icariin on the mRNA and protein expression of NOS isoform in corpus cavernosum of castrated rats[J];Chinese Pharmacological Bulletin;2003-06
6 WANG Yaxuan,CAI Guangzeng,YANG Shuwen,et al.Department of Urology,Second Hospital of Hebei Medical University,Shijiazhuang 050000,China;Study on changes of proliferation and apoptosis of spermatogenic cell in idiopathic oligospermia[J];Chinese Journal of Urology;2002-11
7 HU Zuo-wei, SHEN Zi-yin, HUANG Jian-hua Institute o f Integrative Chinese and Western Medicine, Huashan Hospital, Fudan University, Shanghai(200040);Experimental Study on Effect of Epimedium Flavonoids in Protecting Telo mere Lengt h of Senescence Cells[J];Chinese Journal of Integrated Traditional and Western Medicine;2004-12
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZOU Hao- jun,MING Liang;Progress of studies on anti-inflammation, immunodulatory and anti-senility effects of total flavones[J];Journal of Anhui Health Vocational & Technical College;2003-06
2 ZHU Hai-gui1,Song Xiao-ping2 AnQing Hongxing High School,Department of Biology,Anqing 246300,Anhui;Telomeres and Cellular senescence[J];Journal of Anhui Health Vocational & Technical College;2008-03
3 WANG Dong-mei,DONG Ya-lin,PAN Long,YANG Ping-lin (Dep.of Pharmacology, the First Hospital of Xi'an Jiaotong University, shaanxi Xi'an 710061,China);Influence of Bushen Zhuanggu Granule on the Biochemical Criterion and Bone Density of Primary Osteoporosis in Rats[J];Journal of Anhui Traditional Chinese Medical College;2004-06
4 LIU Hao-ran1,LIU Ying-lin1, PENG Xiang-Dong2, QI Min-you3,WANG Yu-hong4, LI Ya4, LI Yan-lai1 (1 College of Chemical and Chemistry Engineering, Hu’nan University, Changsha 410082,China; 2 The 3rd Hospital of Xiang-ya Medical College, Southern Central University,Changsha 410078,China; 3 College of Pharmacy, Zhe jiang University of Industry, Hangzhou 310014,China; 4 The laboratory of TCM pharmacology and screening for TCM active fractions, Hu'nan University of TCM, Changsha 410007, China);Effects of total flavones of Epimedium brevicornu Maxim on testis diseases in D-galactose-induced aging rats[J];China Journal of Traditional Chinese Medicine and Pharmacy;2009-05
5 ZHANG Xian-ping,WANG Qian-xing,CAI Xiu-lian,LIU Lian (Department of Histology & Embryology,Zunyi Medical College,Zunyi 563003 China);Effect of Manganese on Apoptosis of Spermatogenic Cell of Rats[J];Industrial Health and Occupational Diseases;2008-02
6 DU Peng,XIAO Run-mei,CHEN Yong(School of Life Science,Hubei University,Wuhan 430062,China);Effects of Epimedium sagittatum flavonoids and Eucommia ulmoides flavonoids on osteoporosis mice induced by retinoic acid[J];Journal of Hubei University(Natural Science Edition);2005-04
7 ZHANG Xian-ping,CAI Xiu-lian,WANG Qian-xing,et al.Department of Histology and Embryology,Zunyi Medical College,Zunyi,Guizhou 563003,China;Effect of Manganese on Apoptosis,p53 and Bcl-2 Expression in Spermatogenic Cells of Rats[J];Journal of Environment and Health;2007-04
8 HU Hai-xiang1,LUO Bin2,DONG Jing1(1 General Hospital of Chinese Air Force,Beijing 100036;2 Beijing University of Chinese Medicine);Influence of Bushentongluoshugan Formula on expression of TGF-β mRNA of penile tissue in senescent rats[J];Journal of Beijing University of Traditional Chinese Medicine;2007-10
9 CHEN Feng-zheng,LI Shu-hua (Environment and Life Science Department, Leshan Teachers' College, Leshan 614004, China);Study on the Extractive Technology of Total Flavones from Epimedium Brevicomum Maxim[J];Journal of Leshan Teachers College;2003-04
10 HUANG Yu-feng Laboratory of Reproduction & Genetics,Nanjing General Hospital of Nanjing Command,PLA,Nanjing,Jiangsu 210002,China;Regulation of Spermatogenic Cells Apoptosis by Genes[J];Nationlal Journal of Andrology;2005-09
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 ZHAO Kai,XU Sheng-chun(Dept.of Human Anatomy,Anhui Medical University,Anhui Hefei 230032,China);Effect of Epimedium on Calcium and Phosphorus Metabolism in Degenerated Cervical Vertebral of Rats[J];Journal of Anhui Traditional Chinese Medical College;2008-02
2 WANG Hai-ling1,LIU Ning2,LIU Zhi-qiang2,LIU Zhong-ying1,ZHANG Han-qi3,ZHOU Qiu-li1(1.Department of Biochemistry Analysis,School of Pharmacy,Jilin University,Changchun 130021,China;2.Laboratory of New Drug Research,Changchun Institute of Applied Chemistry,CAS,Changchun 130022,China;3.Department of Chemistry Analysis,School of Chemistry,Jilin University,Changchun 130012,China);Absorption of quercetin and rutin across human intestinal epithelial by using Caco-2 cells[J];Journal of Jilin University(Medicine Edition);2007-01
3 WANG Xue Mei △, FU Hong, LIU Geng Xin (Institute of Integrated of Traditional Chinese and Western Medicine, Peking University, Beijing 100034,China);Effect of Herba Epimedii and Fructus Lycii on mitochondrial DNA deletion, activity of respiratory chain enzyme complexes and ATP synthesis in aged rats[J];Journal of Beijing Medical University;2002-01
4 LI Binbin △, YU Shifeng (Department of Oral Pathology, Peking University School of Stomatology, Beijing 100081,China);Effect of puerarin on the bone metabolism in vitro[J];Journal of Beijing Medical University;2003-01
5 JIANG Kun#, QIN　Yue, TONG Tanjun(#Department of Biochemistry and Molecular Biology,　Beijing Medical University, Beijing 100083);EFFECTS OF erbB-2 REPRESSION AND EGF STIMULATION ON THE LEVEL OF STAT1 AND CYCLIN D1 PROTEIN[J];JOURNAL OF BEIJING MEDICAL UNIVERSITY;1998-03
6 LI Xin,WANG Jin-juan,GAO Fu-lu (Chengde Medical College,Hebei Chengde 067000,China);STUDY ON THE EFFECT OF ZYYSY TO SPERMATOGENESIS DISTURBANCE RATS AND THE EXPRESSION OF P53[J];Journal of Chengde Medical College;2004-01
7 FANG Ze qiang 1,LI Hui zeng 1,WANG Chang yong 2,et al. (1 Department of Stomatology, Southwest Hospital,the Third Military Medical University, Chongqing 400038; 2 Institute of Basic Medical Sciences,the Academy of Military Medical Sciences, Beijing 100850);Experimental study on effect of bcl-2 and p53 on telomerase activity of immortal chondrocytes[J];Chongqing Medical Journal;2003-01
8 Li Shutong; Li Tiejun; Zheng Qinyue; Wang Hongbin (Department of Pharmacology, College of Pharmacy, Second Military Medical University, Shanghai,200433);Effects of icariine on lymphocyte proliferation and production of colony stimulating factor-like activity from mice splenocytes in vitro[J];ACADEMIC JOURNAL OF SECOND MILITARY MEDICAL UNIVERSITY;1995-04
9 Li Shutong;Li Tiejun;Zheng Qinyue;Wang Hongbin(Department of Pharmacology,College of Pharmacy,Second Military Medical University,Shanghai,200433);Effects of icarrin on function of murine peritoneal macrophages[J];ACADEMIC JOURNAL OF SECOND MILITARY MEDICAL UNIVERSITY;1995-06
10 Qin Luping, Shi Hanping, Zheng Shuiqing, Li Bohua, Hong Qiubing, Wang Hongbin, Zhang Jiaqing (Department of Pharmacognosy, College of Pharmacy,Second Military Medicial University, Shanghai, 200433);Effects of osthol and icariin on serum thyroid hormone in mice of hypothyroidism type kidneyyang insufficiency[J];ACADEMIC JOURNAL OF SECOND MILITARY MEDICAL UNIVERSITY;1998-01
【Secondary References】
Chinese Journal Full-text Database 2 Hits
1 WANG Yu,HONG Yuping (The Second People's Hospital of Zhuhai City,Zhuhai 519020,China);Study on extraction of Compound Yinyanghuo Granules[J];China Medical Herald;2009-19
2 Chen Fang,Shen Xiaojun Union Hospital of Tongji Medical College,Huazhong University of Science and Technology(Wuhan 430030);Effect of Icariin on Cell Cycle and Cell Cycle Related Proteins of Vascular Smooth Muscle Cell[J];Chinese Journal of Integrative Medicine on Cardio-/Cerebrovascular Disease;2009-11
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Wang Ruian △, ** Takehiko Koji, *Zheng Pingju, Zhang Yuanqiang, ** Paul K. Nakane (Department of Histology and Embryology,Institute of Basic Medicine; Department of Oncology,Xijing Hospital,The Fourth Military Medical;PROGRAMMED CELL DEATH OF MALE GERM CELLS OF MOUSE DURING POSTNATAL DEVELOPMENT[J];ACTA ANATOMICA SINICA;1998-04
2 Jian Sun TONG 1, Yu Gui CUI 2, Xing Hai WANG 1, Fu Song DI 2, Ding Zhi MA 1, Rui Fen CAI 1, Ting FENG 2, Gui Yuan ZHANG 3 (1. Jiangsu Family Planning Institute, Nanjing,Jiangsu 210029,China; 2. Department of Endocrinology,;Pharmacokinetics and Clinical Study of TU in Patients with Klinefelter′s Syndrome[J];Acto andrologica Sinica;2000-04
3 Chen Guohua, Wang Jianxin. (Fujian Medical University, Fuzhou 350004);Exo-melatonin Influence to the Apoptotic Spermatogenic Cells in the Mice[J];Journal of Qiqihar Medical;2003-10
4 FU Jie, QIAO Liang, JING Tai Yi, LIN Gui Ting, WANG Yin Ye, XIN Zhong Cheng (Institue of Urology , Peking University, Beijing 100034);Effect of Icarrin on cGMP levels in penile corous cavernosum of rabbit[J];Chinese Pharmacological Bulletin;2002-04
5 XIONG Yue-bin; ZHOU Chu-hua(Jiangxi Institute of Medical Sciences),Nanchang 330006;The effect of extracts from herba epimedii and semen cuscutae on thefunction of male reproduction[J];CHINESE PHARMACEUTICAL JOURNAL;1994-02
6 孙丽萍，孙承琳，张丽，刘春梅，葛东宇，张伟;Combined use of Livial,Calcitonin and Nandrolone decanoate to treat osteoporosis in ovariectomized rats[J];Chinese Journal of Osteoporosis;1997-03
7 Liu Kang,Ji Hui,Li Shaoping and Li NaisanDepartment of pharmacology,China Pharmaceutical University,Nanjing 210009,China;Comparison study on three osteoporotic rats models[J];CHINESE JOURNAL OF OSTEOPOROSIS;1998-04
8 Meng Xianli,Zeng Nan,Zhang Yi,Lai Xianrong,Ren Changmao and Cheng Longqiong (Chengdu University of TCM 610075);Studies on Effect of Active Constituents of Herba Epimedii on Hypothalamic Monoamines Neurotransmitter and Other Brain Functions in Aging Rats[J];CHINA JOURNAL OF CHINESE MATERIA MEDICA;1996-11
9 Yang Mei-fang; Li Zhen-jia; Zhu Shou-rong; et al (The PLA General Hospital,Beijing, 100853);THE DEVELOPMENT AND CLINICAL APPLICATION OF A RADIOIMMUNOASSAY FOR SERUM BGP[J];CHINESE JOURNAL OF NUCLEAR MDEICINE;1994-03
10 QIAO Liang,XIN Zhongcheng,FU Jie,et al.Department of Urology,the First Hospital,Institute of Urology,Peking University,Beijing 100034,China;Expression of phosphodiesterase 5 in clitoral corpus cavernosum and effect of icariin on cGMP levels in vitro[J];Chinese Journal of Urology;2002-11
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